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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-21388 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ERH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, H1299, HCT116, mouse brain. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (99%), Xenopus laevis (100%), Pig (100%), Chicken (98%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.79 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ERH Polyclonal Antibody (Product # PA5-21388). Sample (50 µg of whole cell lysate). Lane A: mouse brain . 15% SDS PAGE. ERH Polyclonal Antibody (Product # PA5-21388) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ERH Polyclonal Antibody detects ERH protein by western blot analysis. A. 30 µg A549 whole cell extract. B. 30 µg H1299 whole cell extract. C. 30 µg HCT116 whole cell extract.15 % SDS-PAGE. ERH Polyclonal Antibody (Product # PA5-21388) dilution: 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ERH Polyclonal Antibody detects ERH protein at cytosol on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. ERH Polyclonal Antibody (Product # PA5-21388) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ERH Polyclonal Antibody detects ERH protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. ERH Polyclonal Antibody (Product # PA5-21388) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
