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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA324533 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal UBP1 Antibody (Center)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal UBP1 Antibody (Center)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- UBP1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- UBP1 Antibody (Center) (Cat. #TA324533) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the UBP1 antibody detected the UBP1 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of A549 cell stained with UBP1 Antibody (Center)(Cat#TA324533).A549 cells were incubated with UBP1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml).UBP1 immunoreactivity is localized to Nucleus significantly.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- UBP1 Antibody (Center) (TA324533)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
