A300-814A

antibody from Invitrogen Antibodies

Targeting: TGS1 NCOA6IP, PIMT

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-814A

Provider

Invitrogen Antibodies

Product name

PIMT Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PIMT by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PIMT (Cat. No. A300-814A Lot2) used at a dilution of 1:200 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PIMT by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PIMT antibody A300-814A (lot A300-814A-2) used for IP at 6 µg per reaction. PIMT was also immunoprecipitated by a previous lot of this antibody (lot A300-814A-1) and rabbit anti-PIMT antibody A304-922A. For blotting immunoprecipitated PIMT, A300-814A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.