Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP1-21367 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-21367, RRID:AB_1660521
- Product name
- Rabbit Polyclonal KMT1A/SUV39H1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references SUV39H1 Reduction Is Implicated in Abnormal Inflammation in COPD.
Role of histone lysine methyltransferases SUV39H1 and SETDB1 in gliomagenesis: modulation of cell proliferation, migration, and colony formation.
Activation of Hif1α by the prolylhydroxylase inhibitor dimethyoxalyglycine decreases radiosensitivity.
Chen TT, Wu SM, Ho SC, Chuang HC, Liu CY, Chan YF, Kuo LW, Feng PH, Liu WT, Chen KY, Hsiao TC, Juang JN, Lee KY
Scientific reports 2017 Apr 20;7:46667
Scientific reports 2017 Apr 20;7:46667
Role of histone lysine methyltransferases SUV39H1 and SETDB1 in gliomagenesis: modulation of cell proliferation, migration, and colony formation.
Spyropoulou A, Gargalionis A, Dalagiorgou G, Adamopoulos C, Papavassiliou KA, Lea RW, Piperi C, Papavassiliou AG
Neuromolecular medicine 2014 Mar;16(1):70-82
Neuromolecular medicine 2014 Mar;16(1):70-82
Activation of Hif1α by the prolylhydroxylase inhibitor dimethyoxalyglycine decreases radiosensitivity.
Ayrapetov MK, Xu C, Sun Y, Zhu K, Parmar K, D'Andrea AD, Price BD
PloS one 2011;6(10):e26064
PloS one 2011;6(10):e26064
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - WB analysis of KTM1A/SUV39H1 in Ntera cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - Up: SUV39H1 expression in C6 astrocytic cell line and human glioma cell lines GOS-3, T98G, U87MG and 1321N1. Down: Actin levels. Image submitted by a verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - Up:SUV39H1 expression in normal brain, human glioma tissue grades II, III and IV respectively. Down: Actin expression levels. Image submitted by a verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - Detection of human SUV39H1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SUV39H1 antibody NBP1-21367 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. SUV39H1 was also immunoprecipitated by a rabbit anti-SUV39H1 antibody recognizing a downstream epitope (B). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: KMT1A/SUV39H1 Antibody [NBP1-21367] - IHC analysis of KMT1A/SUV39H1 in human breast cancer using DAB with hematoxylin counterstain.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KMT1A/SUV39H1 Antibody [NBP1-21367] - SUV39H1 expression in human glioma tissue grade IV. Image submitted by a verified customer review.