PA5-29470

antibody from Invitrogen Antibodies

Targeting: SUV39H1 KMT1A, SUV39H

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29470

Provider

Invitrogen Antibodies

Product name

SUV39H1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, H1299, Raji, Mouse brain. Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (90%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.52 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Heterochromatin-Driven Nuclear Softening Protects the Genome against Mechanical Stress-Induced Damage.

Nava MM, Miroshnikova YA, Biggs LC, Whitefield DB, Metge F, Boucas J, Vihinen H, Jokitalo E, Li X, García Arcos JM, Hoffmann B, Merkel R, Niessen CM, Dahl KN, Wickström SA
Cell 2020 May 14;181(4):800-817.e22

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SUV39H1 in paraformaldehyde-fixed HeLa cells using a SUV39H1 polyclonal antibody (Product # PA5-29470) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SUV39H1 (green) in human hepatocytes. The cells were fixed, permeabilized and blocked. Cells were stained with a SUV39H1 polyclonal antibody (Product # PA5-29470) at a dilution of 1:500, followed by staining with Alexa fluor 488 goat anti-rabbit secondary antibody (Product # A-11008) at a dilution of 1:500 (green). Nuclei (blue) were counterstained with DAPI. Images were taken at 50X magnification. Data courtesy of the Antibody Data Exchange Program.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SUV39H1 Polyclonal Antibody detects SUV39H1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SUV39H1 stained by SUV39H1 Polyclonal Antibody (Product # PA5-29470) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 µm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure S4 H3K9me3 Regulates Chromatin Rheology and Nuclear Mechanics, Related to Figure 4 (A) Representative western blots and quantification of GFP-tagged wild-type (WT) Lamin A or Lamin A mutants (Serine 22 to Alanine (S22A) or S22A/S392A double mutant) show equal levels of expression. Lamin A band intensities are normalized to GAPDH (n = 3 independent experiments). (B) Representative immunofluorescence images of nuclei (DAPI), GFP, Lamin A/C, and H3K9me3 in cells expressing WT Lamin A-GFP or Lamin mutants S22A-GFP; S22A/S392A-GFP. (C and D) Quantification of Lamin A intensity and nuclear shape in WT Lamin A and mutant expressing cells (n = 3 independent experiments with > 100 nuclei/condition/experiment; * p = 0.034, ANOVA/Dunnett's). (E) AFM force indentation experiments of cells expressing WT Lamin A-GFP or Lamin mutants S22A-GFP; S22A/S392A-GFP (n = 3 independent experiments with > 40 nuclei/condition/experiment; no statistically significant difference found, Friedman/Dunn's). (F) Representative immunofluorescence images and quantification of Suv39H1 levels in cells exposed to uniaxial 40% stretch for 30 min (n = 3 independent experiments with > 100 nuclei/condition/experiment; * p = 0.05, Mann-Whitney) (G) Quantification of H3K9me3 intensity from cells in (B) (n = 3 independent experiments with > 30 nuclei/condition/experiment; no statistically significant difference found, Friedman/Dunn's). (H) Representative immunofluorescence images and quantification of H3K9me3 lev