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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Flow cytometry [1]
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- Product number
- MAB6947 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB6947, RRID:AB_10680304
- Product name
- KLRD1 monoclonal antibody, clone HP-3D9
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against native KLRD1.
- Isotype
- IgG
- Antibody clone number
- HP-3D9
- Storage
- Store at 4°C.
Submitted references Molecular characterization of human CD94: a type II membrane glycoprotein related to the C-type lectin superfamily.
Human natural killer cell receptors for HLA-class I molecules. Evidence that the Kp43 (CD94) molecule functions as receptor for HLA-B alleles.
A novel functional cell surface dimer (Kp43) expressed by natural killer cells and T cell receptor-gamma/delta+ T lymphocytes. I. Inhibition of the IL-2-dependent proliferation by anti-Kp43 monoclonal antibody.
Chang C, Rodríguez A, Carretero M, López-Botet M, Phillips JH, Lanier LL
European journal of immunology 1995 Sep;25(9):2433-7
European journal of immunology 1995 Sep;25(9):2433-7
Human natural killer cell receptors for HLA-class I molecules. Evidence that the Kp43 (CD94) molecule functions as receptor for HLA-B alleles.
Moretta A, Vitale M, Sivori S, Bottino C, Morelli L, Augugliaro R, Barbaresi M, Pende D, Ciccone E, Lopez-Botet M, Moretta L
The Journal of experimental medicine 1994 Aug 1;180(2):545-55
The Journal of experimental medicine 1994 Aug 1;180(2):545-55
A novel functional cell surface dimer (Kp43) expressed by natural killer cells and T cell receptor-gamma/delta+ T lymphocytes. I. Inhibition of the IL-2-dependent proliferation by anti-Kp43 monoclonal antibody.
Aramburu J, Balboa MA, Ramírez A, Silva A, Acevedo A, Sánchez-Madrid F, De Landázuri MO, López-Botet M
Journal of immunology (Baltimore, Md. : 1950) 1990 Apr 15;144(8):3238-47
Journal of immunology (Baltimore, Md. : 1950) 1990 Apr 15;144(8):3238-47
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Ficoll prepared human peripheral white blood cells were stimulated by incubating 1 day at 5 x 106 cells/mL in RPMI 10% FBS media including 5 ug/mL Phytohemagglutinin-P. 5 X 105 cells per tube were then washed and incubated 45 minutes on ice with 80 uL of KLRD1 monoclonal antibody, clone HP-3D9 (Cat # MAB6947) at a concentration of 20 ug/mL. Cells were washed twice and incubated with 2oreagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A net 40% population of the cells stained positive with a mean shift of 1.10 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
- Validation comment
- Flow Cytometry
