Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-85452 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DUOX1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution. Predicted reactivity: Mouse (89%), Rat (87%), Dog (86%), Pig (90%), Bovine (90%). Positive Control: A549, H1299, HCT116, unboiled A431, A431 membrane extract. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DUOX1 in various whole cell extracts using DUOX1 polyclonal antibody (Product # PA5-85452) using 30 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DUOX1 was performed by separating 30 µg of Boiled and unboiled A431 whole cell and membrane extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a DUOX1 Polyclonal Antibody (Product # PA5-85452) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DUOX1 in paraffin-embedded human gastric cancer tissue using DUOX1 polyclonal antibody (Product # PA5-85452) at a dilution of 1:500.
