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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- R33479 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PDE4B Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids DIDIATEDKSPVDT were used as the immunogen for this PDE4B antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store the PDE4B antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse brain lysate with PDE4B antibody at 0.5ug/ml. Predicted molecular weight: ~83kDa, routinely observed at 83~98kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot of HEK293 lysate overexpressing human PDE4B probed with PDE4B antibody. (mock transfection in lane 1). Predicted molecular weight: ~83kDa, routinely observed at 83~98kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human prostate tissue with PDE4B antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
