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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- PA5-20848 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SIPA1L2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is rat brain tissue lysate.
- Concentration
- 1 mg/mL
Submitted references Endothelial colony-forming cell-derived exosomal miR-21-5p regulates autophagic flux to promote vascular endothelial repair by inhibiting SIPL1A2 in atherosclerosis.
Ke X, Liao Z, Luo X, Chen JQ, Deng M, Huang Y, Wang Z, Wei M
Cell communication and signaling : CCS 2022 Mar 12;20(1):30
Cell communication and signaling : CCS 2022 Mar 12;20(1):30
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain tissue lysate using a SIPA1L2 polyclonal antibody (Product # PA5-20848) at 1 µg/mL in either (A) the presence and (B) the absence of blocking peptide.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SIPA1L2 in rat brain tissue lysate with SIPA1L2 Polyclonal Antibody (Product # PA5-20848) at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of SIPA1L2 in human brain tissue with SIPA1L2 Polyclonal Antibody (Product # PA5-20848) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of SIPA1L2 in human brain tissue with SIPA1L2 Polyclonal Antibody (Product # PA5-20848) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of SIPA1L2 in human brain tissue with SIPA1L2 Polyclonal Antibody (Product # PA5-20848) at 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 7 EPC-exosome-derived miR-21-5p targets the 3'UTR of SIPA1L2. A Venn diagram illustrating the overlapping miR-21-5p-targeted genes predicted using bioinformatics tools (miRanda, starBase, RAID and PITA) and downregulated genes in HMECs following ECFC-exosome treatment. B mRNA and protein expression of SIPA1L2 in HMECs with different treatments was determined by qRT-PCR and western blot assay, respectively. Biological replicates = 3, and technical replicates = 1-3. C The interaction between miR-21-5p and the 3'UTR of SIPA1L2 was evaluated using the dual-luciferase reporter assay. Biological replicates = 5, and technical replicates = 1. D Expression of miR-21-5p and SIPA1L2 in HMECs transfected with miR-21-5p mimics or inhibitor was determined by qRT-PCR. Biological replicates = 3, and technical replicates = 3. E mRNA and protein expression of SIPA1L2 in HMECs with different treatments was determined by qRT-PCR and western blot assay, respectively. Exos ECFC-exosomes, Exos-miR inh ECFC-exosomes were transfected with miR-21-5p inhibitor, Exos-NC inh ECFC-exosomes were transfected with NC inhibitor. Biological replicates = 3, and technical replicates = 1-3. N.S. not significant; significant differences between different treatment groups are indicated as * P < 0.05 and ** P < 0.01
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 9 ECFC-derived exosomes repair vascular injury by rescuing autophagic flux through the miR-21-5p/SIPA1L2 axis in a rat atherosclerosis model. A Expression levels of miR-21-5p and SIPA1L2 in rats with different treatments were determined by qRT-PCR. Biological replicates = 3-6, and technical replicates = 3. B Protein levels of SIPA1L2 and autophagy-related proteins (LC3I, LC3II and p62) in rats with different treatments were determined by qRT-PCR and western blot assay. Biological replicates = 3, and technical replicates = 1. C The schematic diagram illustrates that ECFC-exosomes repair vascular injury by rescuing autophagic flux through the miR-21-5p/SIPA1L2 axis in a rat atherosclerosis model. Exos-miR inh ECFC-exosomes transfected with miR-21-5p inhibitor, Exos-NC inh ECFC-exosomes transfected with NC inhibitor, N.S. not significant; significant differences between different treatment groups are indicated as * P < 0.05 and ** P < 0.01
