PA5-20848

antibody from Invitrogen Antibodies

Targeting: SIPA1L2 KIAA1389, SPAR2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-20848

Provider

Invitrogen Antibodies

Product name

SIPA1L2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is rat brain tissue lysate. PA5-20848 can be used with blocking peptide PEP-0962.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

References

Submitted references

Endothelial colony-forming cell-derived exosomal miR-21-5p regulates autophagic flux to promote vascular endothelial repair by inhibiting SIPL1A2 in atherosclerosis.

Ke X, Liao Z, Luo X, Chen JQ, Deng M, Huang Y, Wang Z, Wei M
Cell communication and signaling : CCS 2022 Mar 12;20(1):30

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain tissue lysate using a SIPA1L2 polyclonal antibody (Product # PA5-20848) at 1 µg/mL in either (A) the presence and (B) the absence of blocking peptide.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SIPA1L2 in rat brain tissue lysate with SIPA1L2 Polyclonal Antibody (Product # PA5-20848) at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 7 EPC-exosome-derived miR-21-5p targets the 3'UTR of SIPA1L2. A Venn diagram illustrating the overlapping miR-21-5p-targeted genes predicted using bioinformatics tools (miRanda, starBase, RAID and PITA) and downregulated genes in HMECs following ECFC-exosome treatment. B mRNA and protein expression of SIPA1L2 in HMECs with different treatments was determined by qRT-PCR and western blot assay, respectively. Biological replicates = 3, and technical replicates = 1-3. C The interaction between miR-21-5p and the 3'UTR of SIPA1L2 was evaluated using the dual-luciferase reporter assay. Biological replicates = 5, and technical replicates = 1. D Expression of miR-21-5p and SIPA1L2 in HMECs transfected with miR-21-5p mimics or inhibitor was determined by qRT-PCR. Biological replicates = 3, and technical replicates = 3. E mRNA and protein expression of SIPA1L2 in HMECs with different treatments was determined by qRT-PCR and western blot assay, respectively. Exos ECFC-exosomes, Exos-miR inh ECFC-exosomes were transfected with miR-21-5p inhibitor, Exos-NC inh ECFC-exosomes were transfected with NC inhibitor. Biological replicates = 3, and technical replicates = 1-3. N.S. not significant; significant differences between different treatment groups are indicated as * P < 0.05 and ** P < 0.01

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 9 ECFC-derived exosomes repair vascular injury by rescuing autophagic flux through the miR-21-5p/SIPA1L2 axis in a rat atherosclerosis model. A Expression levels of miR-21-5p and SIPA1L2 in rats with different treatments were determined by qRT-PCR. Biological replicates = 3-6, and technical replicates = 3. B Protein levels of SIPA1L2 and autophagy-related proteins (LC3I, LC3II and p62) in rats with different treatments were determined by qRT-PCR and western blot assay. Biological replicates = 3, and technical replicates = 1. C The schematic diagram illustrates that ECFC-exosomes repair vascular injury by rescuing autophagic flux through the miR-21-5p/SIPA1L2 axis in a rat atherosclerosis model. Exos-miR inh ECFC-exosomes transfected with miR-21-5p inhibitor, Exos-NC inh ECFC-exosomes transfected with NC inhibitor, N.S. not significant; significant differences between different treatment groups are indicated as * P < 0.05 and ** P < 0.01