Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB300-840 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-840, RRID:AB_2210773
- Product name
- Goat Polyclonal UBE2K/E2-25K Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise isoform 1 (NP_005330.1), isoform 2 (NP_001104582.1) and isoform 3 (NP_001104583.1).
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme.
Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme.
Kalchman MA, Graham RK, Xia G, Koide HB, Hodgson JG, Graham KC, Goldberg YP, Gietz RD, Pickart CM, Hayden MR
The Journal of biological chemistry 1996 Aug 9;271(32):19385-94
The Journal of biological chemistry 1996 Aug 9;271(32):19385-94
Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme.
Kalchman MA, Graham RK, Xia G, Koide HB, Hodgson JG, Graham KC, Goldberg YP, Gietz RD, Pickart CM, Hayden MR
The Journal of biological chemistry 1996 Aug 9;271(32):19385-94
The Journal of biological chemistry 1996 Aug 9;271(32):19385-94
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UBE2K/E2-25K Antibody [NB300-840] - (1.5ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UBE2K/E2-25K Antibody [NB300-840] - (3.8ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6,