Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 15354-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#15354-1-AP, RRID:AB_2147773
- Product name
- MYL9 antibody
- Antibody type
- Polyclonal
- Description
- MYL9 antibody (Cat. #15354-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Transfer of Damaged Mitochondria from Cancer Cells to Cancer-Associated Fibroblasts Promotes Tyrosine Kinase Inhibitor Tolerance in EGFR-Mutant Lung Cancer.
Metabolite-gated vascular contractility switch: OXGR1 activation mechanism enables agonist therapy for rosacea erythema.
RNA binding protein DDX3X drives pancreatic cancer progression via the TLE2-MYL9 axis.
Ethanolic Extract of Atractylodis Rhizoma Ameliorates DSS-Induced Ulcerative Colitis via Improving Intestinal Barrier Function Through the Rho-Associated Kinase/Myosin Light Chain Pathway.
SOX17 Prevents Endothelial-Mesenchymal Transition of Pulmonary Arterial Endothelial Cells in Pulmonary Hypertension through Mediating TGF-β/Smad2/3 Signaling.
Inhibition of Rho GEFs attenuates pulmonary fibrosis through suppressing myofibroblast activation and reprogramming profibrotic macrophages.
Chronic Stress-induced Serotonin Impairs Intestinal Epithelial Cell Mitochondrial Biogenesis via the AMPK-PGC-1α Axis.
Single-cell transcriptomics reveals aberrant skin-resident cell populations and identifies fibroblasts as a determinant in rosacea.
A Potential Use of Vidarabine: Alleviation of Functional Constipation Through Modulation of the Adenosine A2A Receptor-MLC Signaling Pathway and the Gut Microbiota.
Network pharmacology combined with an animal model to reveal the material basis and mechanism of Amomum villosum in alleviating constipation in mice.
CSE1L in enhancing the effect of defactinib on gastric cancer cells via the inhibition of FAK phosphorylation.
PRPF19 facilitates colorectal cancer liver metastasis through activation of the Src-YAP1 pathway via K63-linked ubiquitination of MYL9.
Paracrine HB-EGF signaling reduce enhanced contractile and energetic state of activated decidual fibroblasts by rebalancing SRF-MRTF-TCF transcriptional axis.
Proteomic profiling of concurrently isolated primary microvascular endothelial cells, pericytes, and vascular smooth muscle cells from adult mouse heart.
Brain-derived neurotrophic factor regulates LYN kinase-mediated myosin light chain kinase activation to modulate nonmuscle myosin II activity in hippocampal neurons.
Transient expansion and myofibroblast conversion of adipogenic lineage precursors mediate bone marrow repair after radiation.
Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer.
Fenretinide inhibits the proliferation and migration of human liver cancer HepG2 cells by downregulating the activation of myosin light chain kinase through the p38‑MAPK signaling pathway.
Liu T, Huang S, Li Z, Li J, Sun Q, Wang Q, Shen Z, Xia Z, Meng F, Xu Y, Xia W, Tan J, Yin R
Cancer research 2026 Mar 2;86(5):1215-1231
Cancer research 2026 Mar 2;86(5):1215-1231
Metabolite-gated vascular contractility switch: OXGR1 activation mechanism enables agonist therapy for rosacea erythema.
Xiao W, Zhu Y, Tang X, Zhu K, Zhang W, Chen M, Cai K, Xu S, Wu Z, Wang M, Liu J, Long L, Tan Z, Wu A, Zhou S, Zhao Z, Tang Y, Huang Y, Wang B, Liu F, Wang Q, Yang F, Jian D, Shi W, Xie H, Chen X, Guo L, Deng Z, Sun J, Li J
Cell 2026 Apr 2;189(7):1990-2006.e30
Cell 2026 Apr 2;189(7):1990-2006.e30
RNA binding protein DDX3X drives pancreatic cancer progression via the TLE2-MYL9 axis.
Wang Y, Yang Q, Lin F, Song X, Yang G, Wen D, Yang Y, Wu B, Meng Y, Zhang N, Lu X, Xiong C, Zhao W, Liang J, Zhang T, Liu Y
Science advances 2025 Sep 12;11(37):eadw9519
Science advances 2025 Sep 12;11(37):eadw9519
Ethanolic Extract of Atractylodis Rhizoma Ameliorates DSS-Induced Ulcerative Colitis via Improving Intestinal Barrier Function Through the Rho-Associated Kinase/Myosin Light Chain Pathway.
Gao Z, Huang P, Yu X, Dong R, Lin Y, Tu J, Chen L, Cao Y, Liu Y, Yang D, Cao G
Journal of medicinal food 2025 Jul;28(7):694-707
Journal of medicinal food 2025 Jul;28(7):694-707
SOX17 Prevents Endothelial-Mesenchymal Transition of Pulmonary Arterial Endothelial Cells in Pulmonary Hypertension through Mediating TGF-β/Smad2/3 Signaling.
Zou X, Yuan M, Zhou W, Cai A, Cheng Y, Zhan Z, Zhang Y, Pan Z, Hu X, Zheng S, Liu T, Huang P
American journal of respiratory cell and molecular biology 2025 Apr;72(4):364-379
American journal of respiratory cell and molecular biology 2025 Apr;72(4):364-379
Inhibition of Rho GEFs attenuates pulmonary fibrosis through suppressing myofibroblast activation and reprogramming profibrotic macrophages.
Luo C, Huang C, Zhu Y, Zhou Y, Qiao Y, Shi C, Gao Y, Guo Y, Wei L
Cell death & disease 2025 Apr 11;16(1):278
Cell death & disease 2025 Apr 11;16(1):278
Chronic Stress-induced Serotonin Impairs Intestinal Epithelial Cell Mitochondrial Biogenesis via the AMPK-PGC-1α Axis.
Yang D, Sun Y, Wen P, Chen Y, Cao J, Sun X, Dong Y
International journal of biological sciences 2024;20(11):4476-4495
International journal of biological sciences 2024;20(11):4476-4495
Single-cell transcriptomics reveals aberrant skin-resident cell populations and identifies fibroblasts as a determinant in rosacea.
Chen M, Yang L, Zhou P, Jin S, Wu Z, Tan Z, Xiao W, Xu S, Zhu Y, Wang M, Jian D, Liu F, Tang Y, Zhao Z, Huang Y, Shi W, Xie H, Nie Q, Wang B, Deng Z, Li J
Nature communications 2024 Oct 9;15(1):8737
Nature communications 2024 Oct 9;15(1):8737
A Potential Use of Vidarabine: Alleviation of Functional Constipation Through Modulation of the Adenosine A2A Receptor-MLC Signaling Pathway and the Gut Microbiota.
Gao X, Guo K, Liu S, Yang W, Sheng J, Tian Y, Peng L, Zhao Y
International journal of molecular sciences 2024 Nov 28;25(23)
International journal of molecular sciences 2024 Nov 28;25(23)
Network pharmacology combined with an animal model to reveal the material basis and mechanism of Amomum villosum in alleviating constipation in mice.
Liu S, Zhao Y, Li S, Li Y, Liu L, Sheng J, Tian Y, Gao X
Gene 2024 Mar 1;897:148064
Gene 2024 Mar 1;897:148064
CSE1L in enhancing the effect of defactinib on gastric cancer cells via the inhibition of FAK phosphorylation.
He X, Wang Y, Zhang Y, Sahu A, Almhanna K, Liu Y
Translational cancer research 2024 Dec 31;13(12):6905-6918
Translational cancer research 2024 Dec 31;13(12):6905-6918
PRPF19 facilitates colorectal cancer liver metastasis through activation of the Src-YAP1 pathway via K63-linked ubiquitination of MYL9.
Zhou R, Chen J, Xu Y, Ye Y, Zhong G, Chen T, Qiu L
Cell death & disease 2023 Apr 8;14(4):258
Cell death & disease 2023 Apr 8;14(4):258
Paracrine HB-EGF signaling reduce enhanced contractile and energetic state of activated decidual fibroblasts by rebalancing SRF-MRTF-TCF transcriptional axis.
Afzal J, Du W, Novin A, Liu Y, Wali K, Murthy A, Garen A, Wagner G, Kshitiz
Frontiers in cell and developmental biology 2022;10:927631
Frontiers in cell and developmental biology 2022;10:927631
Proteomic profiling of concurrently isolated primary microvascular endothelial cells, pericytes, and vascular smooth muscle cells from adult mouse heart.
Cao Z, Minnier J, Liu L, Scott KLL, Reddy AP, Wilmarth PA, David LL, Barnes AP, Grafe MR, Kaul S, Alkayed NJ, Davis CM
Scientific reports 2022 May 25;12(1):8835
Scientific reports 2022 May 25;12(1):8835
Brain-derived neurotrophic factor regulates LYN kinase-mediated myosin light chain kinase activation to modulate nonmuscle myosin II activity in hippocampal neurons.
Li X, Yuan RR, Wang Q, Chai S, Zhang Z, Wang Y, Huang SH
The Journal of biological chemistry 2022 Jun;298(6):102054
The Journal of biological chemistry 2022 Jun;298(6):102054
Transient expansion and myofibroblast conversion of adipogenic lineage precursors mediate bone marrow repair after radiation.
Zhong L, Yao L, Holdreith N, Yu W, Gui T, Miao Z, Elkaim Y, Li M, Gong Y, Pacifici M, Maity A, Busch TM, Joeng KS, Cengel K, Seale P, Tong W, Qin L
JCI insight 2022 Apr 8;7(7)
JCI insight 2022 Apr 8;7(7)
Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer.
He F, Ding H, Zhou Y, Wang Y, Xie J, Yang S, Zhu Y
Frontiers in medicine 2021;8:792740
Frontiers in medicine 2021;8:792740
Fenretinide inhibits the proliferation and migration of human liver cancer HepG2 cells by downregulating the activation of myosin light chain kinase through the p38‑MAPK signaling pathway.
Zhang L, Huang D, Shao D, Liu H, Zhou Q, Gui S, Wei W, Wang Y
Oncology reports 2018 Jul;40(1):518-526
Oncology reports 2018 Jul;40(1):518-526
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human colon tissue were subjected to SDS PAGE followed by western blot with 15354-1-AP(MYL9 antibody) at dilution of 1:400
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using MYL9 antibody 15354-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human colon cancer using 15354-1-AP(MYL9 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human colon cancer using 15354-1-AP(MYL9 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
