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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- TA319521 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-Myosin antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-Myosin antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- Myl9
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.71 mg/mL
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- WB using anti-RLC of Smooth and Non-muscle Myosin antibody to detect vascular myosin (rat aorta, lane 1) but not cardiac myosin (mouse heart, lane2).The primary antibody was diluted in blocking buffer to 1:600 for 2 h at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800? conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at RT. Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
- Validation comment
- WB
