PAB11255

antibody from Abnova Corporation

Targeting: MYL9 LC20, MLC2, MRLC1, MYRL2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB11255

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB11255, RRID:AB_1676360

Product name

MYL9 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of MYL9.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Diphosphorylated MRLC is required for organization of stress fibers in interphase cells and the contractile ring in dividing cells.

Iwasaki T, Murata-Hori M, Ishitobi S, Hosoya H
Cell structure and function 2001 Dec;26(6):677-83

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Characterization and differential expression of human vascular smooth muscle myosin light chain 2 isoform in nonmuscle cells.

Kumar CC, Mohan SR, Zavodny PJ, Narula SK, Leibowitz PJ
Biochemistry 1989 May 2;28(9):4027-35

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using MYL9 polyclonal antibody (Cat # PAB11255) to detect vascular myosin (rat aorta, lane 1), but not cardiac myosin (mouse heart, lane2).Each lanewas loaded with 35 ug of lysate.Arrowheads indicate the detection of both mono-phosphorylated (upper) and unphosphorylated (lower) forms of the protein.After blocking with 5% NGS and 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted in blocking buffer to1 : 600 for 2 h at room temperature.The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye 800™ conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800nm channel, green).Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).IRDye™ 800 fluorescence image was captured usingthe Odyssey® Infrared Imaging System developedby LI-COR.IRDye is a trademark of LI-COR, Inc.