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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- PA5-17726 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-MYL9 (Ser19) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody. This antibody is not cross-reactive with the cardiac isoform of myosin light chain 2.
- Reactivity
- Human, Mouse, Rat, Drosophila
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 103 µg/mL
- Storage
- -20°C
Submitted references DJ4 Targets the Rho-Associated Protein Kinase Pathway and Attenuates Disease Progression in Preclinical Murine Models of Acute Myeloid Leukemia.
IL-9 Abrogates the Metastatic Potential of Breast Cancer by Controlling Extracellular Matrix Remodeling and Cellular Contractility.
Hydrostatic mechanical stress regulates growth and maturation of the atrioventricular valve.
Anillin controls cleavage furrow formation in the course of asymmetric division during mouse oocyte maturation.
Golla U, Ehudin MA, Annageldiyev C, Zeng Z, Bastihalli Tukaramrao D, Tarren A, Date AA, Elcheva I, Berg A, Amin S, Loughran TP Jr, Kester M, Desai D, Dovat S, Claxton D, Sharma A
Cancers 2021 Sep 29;13(19)
Cancers 2021 Sep 29;13(19)
IL-9 Abrogates the Metastatic Potential of Breast Cancer by Controlling Extracellular Matrix Remodeling and Cellular Contractility.
Das S, Surve V, Marathe S, Wad S, Karulkar A, Srinivasan S, Dwivedi A, Barthel SR, Purwar R
Journal of immunology (Baltimore, Md. : 1950) 2021 Jun 1;206(11):2740-2752
Journal of immunology (Baltimore, Md. : 1950) 2021 Jun 1;206(11):2740-2752
Hydrostatic mechanical stress regulates growth and maturation of the atrioventricular valve.
Bassen D, Wang M, Pham D, Sun S, Rao R, Singh R, Butcher J
Development (Cambridge, England) 2021 Jul 1;148(13)
Development (Cambridge, England) 2021 Jul 1;148(13)
Anillin controls cleavage furrow formation in the course of asymmetric division during mouse oocyte maturation.
Lee SR, Jo YJ, Namgoong S, Kim NH
Molecular reproduction and development 2016 Sep;83(9):792-801
Molecular reproduction and development 2016 Sep;83(9):792-801
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-MYL9 (Ser19) using a polyclonal antibody (Product # PA5-17726).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-Myosin Light Chain 2 pSer19 in HeLa cells, phosphatase-treated, using a Phospho-Myosin Light Chain 2 pSer19 polyclonal antibody (Product # PA5-17726) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 DJ4 effectively inhibits downstream ROCK substrates in human AML cell lines. Immunoblot analysis of the relative activity of MYPT1, MLC2, and expression levels of ROCK1, and ROCK2 upon treating the whole cell lysates of ( A ) MV4-11 and ( B ) OCI-AML3 with DJ4 for 24 h. The phosphorylated levels of MYPT1 and MLC2 relative to the loading control GAPDH in ( C ) MV4-11 and ( D ) OCI-AML3 in the presence of DJ4 were quantified. The data were representative of three independent experiments. The results were assessed by means of the unpaired t -test and * p < 0.05, ** p < 0.01, and *** p < 0.005 were considered significant. The data are the means +- SEM ( n = 3).
