MA5-15163

antibody from Invitrogen Antibodies

Targeting: MYL9 LC20, MLC2, MRLC1, MYRL2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15163

Provider

Invitrogen Antibodies

Product name

Phospho-MYL9 (Ser19) Monoclonal Antibody (R.179.1)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody.

Antibody clone number

R.179.1

Concentration

445 µg/mL

References

Submitted references

Behind Brain Metastases Formation: Cellular and Molecular Alterations and Blood-Brain Barrier Disruption.

Godinho-Pereira J, Garcia AR, Figueira I, Malhó R, Brito MA
International journal of molecular sciences 2021 Jun 30;22(13)

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ADF and cofilin-1 collaborate to promote cortical actin flow and the leader bleb-based migration of confined cells.

Ullo MF, Logue JS
eLife 2021 Jun 25;10

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Switching between blebbing and lamellipodia depends on the degree of non-muscle myosin II activity.

Ghosh I, Singh RK, Mishra M, Kapoor S, Jana SS
Journal of cell science 2021 Jan 13;134(1)

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Doxorubicin induces detrusor smooth muscle impairments through myosin dysregulation, leading to a risk of lower urinary tract dysfunction.

Iguchi N, Dönmez Mİ, Carrasco A Jr, Wilcox DT, Pineda RH, Malykhina AP, Cost NG
American journal of physiology. Renal physiology 2019 Jul 1;317(1):F197-F206

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Septin-dependent remodeling of cortical microtubule drives cell reshaping during epithelial wound healing.

Shindo A, Audrey A, Takagishi M, Takahashi M, Wallingford JB, Kinoshita M
Journal of cell science 2018 Jun 28;131(12)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-Myosin Light Chain 2 pSer19 in extracts from HEK293 cells, untreated (-) or lambda phosphatase-treated (+), using Phospho-Myosin Light Chain 2 pSer19 monoclonal antibody (Product # MA5-15163) (upper) or Phospho-Myosin Light Chain 2 (Ser19) monoclonal antibody (lower).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Phospho-MYL9 (Ser19) Monoclonal Antibody (R.179.1) (Product # MA5-15163) and a ~20 kDa band corresponding to Myosin regulatory light polypeptide 9 was observed in C2C12 after treatment with Calyculin A. Whole cell extracts (40 µg lysate) of C2C12 (Lane 1) and Calyculin A treated C2C12 (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:20000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Phospho-Myosin Light Chain 2 pSer19 in HeLa cells using a Phospho-Myosin Light Chain 2 pSer19 onoclonal antibody (Product # MA5-15163) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.