LS-B2794

antibody from LSBio

Targeting: PAG1 CBP, PAG

Western blot (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-B2794

Provider

LSBio

Proper citation

LifeSpan Cat#LS-B2794, RRID:AB_2283502

Product name

IHC-plus™ PAG1 Antibody (C-Terminus, clone PAG-C1) LS-B2794

Antibody type

Monoclonal

Description

Purified by protein-A affinity chromatography. Greater than 95% by SDS-PAGE.

Reactivity

Human, Mouse, Rat, Bovine

Host

Mouse

Isotype

IgG

Antibody clone number

PAG-C1

Storage

Store at 2°C to 8°C. Do not freeze.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitation of human PAG/Cbp from the lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-human PAG (MEM-255). Note: PAG/Cbp is a 46 kDa adaptor protein, which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Lane 1,2: immunoprecipitation with anti-PAG (PAG-C1) Lane 3: immunoprecipitation with anti-PAG (polyclonal antibody)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation of human PAG/Cbp from the lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-human PAG (MEM-255). Note: PAG/Cbp is a 46 kDa adaptor protein, which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Lane 1,2: immunoprecipitation with anti-PAG (PAG-C1) Lane 3: immunoprecipitation with anti-PAG (polyclonal antibody)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation of human PAG/Cbp from the lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-human PAG (MEM-255). Note: PAG/Cbp is a 46 kDa adaptor protein, which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Lane 1,2: immunoprecipitation with anti-PAG (PAG-C1) Lane 3: immunoprecipitation with anti-PAG (polyclonal antibody)

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-PAG1 / PAG antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.