Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PA1-19375 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PAG1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- This antibody recognizes intracellular part of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blotting analysis of human PAG using rabbit polyclonal antibody PAb (409) (Product # PA1-19375) on lysates of Daudi and Ramos cell line under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of rabbit anti-PAG monoclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. PAG was detected around 65kDa.