Antibody data
- Antibody Data
- Antigen structure
- References [31]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 10233-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#10233-1-AP, RRID:AB_2149877
- Product name
- NDE1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated NDE1 antibody (Cat. #10233-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Behavioral Deficits in Mice with Postnatal Disruption of Ndel1 in Forebrain Excitatory Neurons: Implications for Epilepsy and Neuropsychiatric Disorders.
Cilium induction triggers differentiation of glioma stem cells.
FBW7 couples structural integrity with functional output of primary cilia.
Phosphorylation and Pin1 binding to the LIC1 subunit selectively regulate mitotic dynein functions.
Loss of polycystins suppresses deciliation via the activation of the centrosomal integrity pathway.
CENP-F stabilizes kinetochore-microtubule attachments and limits dynein stripping of corona cargoes.
Phenylephrine alleviates 131I damage in submandibular gland through promoting endogenous stem cell regeneration via lissencephaly-1 upregulation.
The DISC1-Girdin complex - a missing link in signaling to the T cell cytoskeleton.
NRF2 negatively regulates primary ciliogenesis and hedgehog signaling.
Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.
Neddylation inhibitor MLN4924 suppresses cilia formation by modulating AKT1.
A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.
Extraciliary roles of the ciliopathy protein JBTS17 in mitosis and neurogenesis.
NDE1 positively regulates oligodendrocyte morphological differentiation.
The LIS1/NDE1 Complex Is Essential for FGF Signaling by Regulating FGF Receptor Intracellular Trafficking.
Lateral attachment of kinetochores to microtubules is enriched in prometaphase rosette and facilitates chromosome alignment and bi-orientation establishment.
The NDE1 genomic locus can affect treatment of psychiatric illness through gene expression changes related to microRNA-484.
N-Acetyl-D-Glucosamine Kinase Interacts with Dynein-Lis1-NudE1 Complex and Regulates Cell Division.
Dynein Separately Partners with NDE1 and Dynactin To Orchestrate T Cell Focused Secretion.
NDE1 and GSK3β Associate with TRAK1 and Regulate Axonal Mitochondrial Motility: Identification of Cyclic AMP as a Novel Modulator of Axonal Mitochondrial Trafficking.
Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.
CPAP promotes timely cilium disassembly to maintain neural progenitor pool.
Cell cycle-dependent ubiquitylation and destruction of NDE1 by CDK5-FBW7 regulates ciliary length.
The scaffold protein Nde1 safeguards the brain genome during S phase of early neural progenitor differentiation.
The essential role of centrosomal NDE1 in human cerebral cortex neurogenesis.
Nde1-mediated inhibition of ciliogenesis affects cell cycle re-entry.
Phosphodiesterase signaling system is disrupted in the cerebella of subjects with schizophrenia, bipolar disorder, and major depression.
Functional interplay between LIS1, NDE1 and NDEL1 in dynein-dependent organelle positioning.
Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly.
NDE1 and NDEL1: multimerisation, alternate splicing and DISC1 interaction.
Cenp-F links kinetochores to Ndel1/Nde1/Lis1/dynein microtubule motor complexes.
Gavrilovici C, Jiang Y, Kiroski I, Sterley TL, Vandal M, Bains J, Park SK, Rho JM, Teskey GC, Nguyen MD
Cerebral cortex communications 2021;2(1):tgaa096
Cerebral cortex communications 2021;2(1):tgaa096
Cilium induction triggers differentiation of glioma stem cells.
Goranci-Buzhala G, Mariappan A, Ricci-Vitiani L, Josipovic N, Pacioni S, Gottardo M, Ptok J, Schaal H, Callaini G, Rajalingam K, Dynlacht B, Hadian K, Papantonis A, Pallini R, Gopalakrishnan J
Cell reports 2021 Sep 7;36(10):109656
Cell reports 2021 Sep 7;36(10):109656
FBW7 couples structural integrity with functional output of primary cilia.
Petsouki E, Gerakopoulos V, Szeto N, Chang W, Humphrey MB, Tsiokas L
Communications biology 2021 Sep 13;4(1):1066
Communications biology 2021 Sep 13;4(1):1066
Phosphorylation and Pin1 binding to the LIC1 subunit selectively regulate mitotic dynein functions.
Kumari A, Kumar C, Pergu R, Kumar M, Mahale SP, Wasnik N, Mylavarapu SVS
The Journal of cell biology 2021 Dec 6;220(12)
The Journal of cell biology 2021 Dec 6;220(12)
Loss of polycystins suppresses deciliation via the activation of the centrosomal integrity pathway.
Gerakopoulos V, Ngo P, Tsiokas L
Life science alliance 2020 Sep;3(9)
Life science alliance 2020 Sep;3(9)
CENP-F stabilizes kinetochore-microtubule attachments and limits dynein stripping of corona cargoes.
Auckland P, Roscioli E, Coker HLE, McAinsh AD
The Journal of cell biology 2020 May 4;219(5)
The Journal of cell biology 2020 May 4;219(5)
Phenylephrine alleviates 131I damage in submandibular gland through promoting endogenous stem cell regeneration via lissencephaly-1 upregulation.
Wang XY, Yu J, Zhang Y, Zhang FY, Liu KJ, Xiang B
Toxicology and applied pharmacology 2020 Jun 1;396:114999
Toxicology and applied pharmacology 2020 Jun 1;396:114999
The DISC1-Girdin complex - a missing link in signaling to the T cell cytoskeleton.
Maskalenko N, Nath S, Ramakrishnan A, Anikeeva N, Sykulev Y, Poenie M
Journal of cell science 2020 Jul 6;133(13)
Journal of cell science 2020 Jul 6;133(13)
NRF2 negatively regulates primary ciliogenesis and hedgehog signaling.
Liu P, Dodson M, Fang D, Chapman E, Zhang DD
PLoS biology 2020 Feb;18(2):e3000620
PLoS biology 2020 Feb;18(2):e3000620
Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.
Kodani A, Kenny C, Lai A, Gonzalez DM, Stronge E, Sejourne GM, Isacco L, Partlow JN, O'Donnell A, McWalter K, Byrne AB, Barkovich AJ, Yang E, Hill RS, Gawlinski P, Wiszniewski W, Cohen JS, Fatemi SA, Baranano KW, Sahin M, Vossler DG, Yuskaitis CJ, Walsh CA
Neuron 2020 Apr 22;106(2):246-255.e6
Neuron 2020 Apr 22;106(2):246-255.e6
Neddylation inhibitor MLN4924 suppresses cilia formation by modulating AKT1.
Mao H, Tang Z, Li H, Sun B, Tan M, Fan S, Zhu Y, Sun Y
Protein & cell 2019 Oct;10(10):726-744
Protein & cell 2019 Oct;10(10):726-744
A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.
So C, Seres KB, Steyer AM, Mönnich E, Clift D, Pejkovska A, Möbius W, Schuh M
Science (New York, N.Y.) 2019 Jun 28;364(6447)
Science (New York, N.Y.) 2019 Jun 28;364(6447)
Extraciliary roles of the ciliopathy protein JBTS17 in mitosis and neurogenesis.
Hong H, Joo K, Park SM, Seo J, Kim MH, Shin E, Cheong HI, Lee JH, Kim J
Annals of neurology 2019 Jul;86(1):99-115
Annals of neurology 2019 Jul;86(1):99-115
NDE1 positively regulates oligodendrocyte morphological differentiation.
Shimizu S, Ishino Y, Tohyama M, Miyata S
Scientific reports 2018 May 16;8(1):7644
Scientific reports 2018 May 16;8(1):7644
The LIS1/NDE1 Complex Is Essential for FGF Signaling by Regulating FGF Receptor Intracellular Trafficking.
Liu L, Lu J, Li X, Wu A, Wu Q, Zhao M, Tang N, Song H
Cell reports 2018 Mar 20;22(12):3277-3291
Cell reports 2018 Mar 20;22(12):3277-3291
Lateral attachment of kinetochores to microtubules is enriched in prometaphase rosette and facilitates chromosome alignment and bi-orientation establishment.
Itoh G, Ikeda M, Iemura K, Amin MA, Kuriyama S, Tanaka M, Mizuno N, Osakada H, Haraguchi T, Tanaka K
Scientific reports 2018 Mar 1;8(1):3888
Scientific reports 2018 Mar 1;8(1):3888
The NDE1 genomic locus can affect treatment of psychiatric illness through gene expression changes related to microRNA-484.
Bradshaw NJ, Ukkola-Vuoti L, Pankakoski M, Zheutlin AB, Ortega-Alonso A, Torniainen-Holm M, Sinha V, Therman S, Paunio T, Suvisaari J, Lönnqvist J, Cannon TD, Haukka J, Hennah W
Open biology 2017 Nov;7(11)
Open biology 2017 Nov;7(11)
N-Acetyl-D-Glucosamine Kinase Interacts with Dynein-Lis1-NudE1 Complex and Regulates Cell Division.
Sharif SR, Islam A, Moon IS
Molecules and cells 2016 Sep;39(9):669-79
Molecules and cells 2016 Sep;39(9):669-79
Dynein Separately Partners with NDE1 and Dynactin To Orchestrate T Cell Focused Secretion.
Nath S, Christian L, Tan SY, Ki S, Ehrlich LI, Poenie M
Journal of immunology (Baltimore, Md. : 1950) 2016 Sep 15;197(6):2090-101
Journal of immunology (Baltimore, Md. : 1950) 2016 Sep 15;197(6):2090-101
NDE1 and GSK3β Associate with TRAK1 and Regulate Axonal Mitochondrial Motility: Identification of Cyclic AMP as a Novel Modulator of Axonal Mitochondrial Trafficking.
Ogawa F, Murphy LC, Malavasi EL, O'Sullivan ST, Torrance HS, Porteous DJ, Millar JK
ACS chemical neuroscience 2016 May 18;7(5):553-64
ACS chemical neuroscience 2016 May 18;7(5):553-64
Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.
Inaba H, Goto H, Kasahara K, Kumamoto K, Yonemura S, Inoko A, Yamano S, Wanibuchi H, He D, Goshima N, Kiyono T, Hirotsune S, Inagaki M
The Journal of cell biology 2016 Feb 15;212(4):409-23
The Journal of cell biology 2016 Feb 15;212(4):409-23
CPAP promotes timely cilium disassembly to maintain neural progenitor pool.
Gabriel E, Wason A, Ramani A, Gooi LM, Keller P, Pozniakovsky A, Poser I, Noack F, Telugu NS, Calegari F, Šarić T, Hescheler J, Hyman AA, Gottardo M, Callaini G, Alkuraya FS, Gopalakrishnan J
The EMBO journal 2016 Apr 15;35(8):803-19
The EMBO journal 2016 Apr 15;35(8):803-19
Cell cycle-dependent ubiquitylation and destruction of NDE1 by CDK5-FBW7 regulates ciliary length.
Maskey D, Marlin MC, Kim S, Kim S, Ong EC, Li G, Tsiokas L
The EMBO journal 2015 Oct 1;34(19):2424-40
The EMBO journal 2015 Oct 1;34(19):2424-40
The scaffold protein Nde1 safeguards the brain genome during S phase of early neural progenitor differentiation.
Houlihan SL, Feng Y
eLife 2014 Sep 23;3:e03297
eLife 2014 Sep 23;3:e03297
The essential role of centrosomal NDE1 in human cerebral cortex neurogenesis.
Bakircioglu M, Carvalho OP, Khurshid M, Cox JJ, Tuysuz B, Barak T, Yilmaz S, Caglayan O, Dincer A, Nicholas AK, Quarrell O, Springell K, Karbani G, Malik S, Gannon C, Sheridan E, Crosier M, Lisgo SN, Lindsay S, Bilguvar K, Gergely F, Gunel M, Woods CG
American journal of human genetics 2011 May 13;88(5):523-35
American journal of human genetics 2011 May 13;88(5):523-35
Nde1-mediated inhibition of ciliogenesis affects cell cycle re-entry.
Kim S, Zaghloul NA, Bubenshchikova E, Oh EC, Rankin S, Katsanis N, Obara T, Tsiokas L
Nature cell biology 2011 Apr;13(4):351-60
Nature cell biology 2011 Apr;13(4):351-60
Phosphodiesterase signaling system is disrupted in the cerebella of subjects with schizophrenia, bipolar disorder, and major depression.
Fatemi SH, Folsom TD, Reutiman TJ, Vazquez G
Schizophrenia research 2010 Jun;119(1-3):266-7
Schizophrenia research 2010 Jun;119(1-3):266-7
Functional interplay between LIS1, NDE1 and NDEL1 in dynein-dependent organelle positioning.
Lam C, Vergnolle MA, Thorpe L, Woodman PG, Allan VJ
Journal of cell science 2010 Jan 15;123(Pt 2):202-12
Journal of cell science 2010 Jan 15;123(Pt 2):202-12
Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly.
Chan YW, Fava LL, Uldschmid A, Schmitz MH, Gerlich DW, Nigg EA, Santamaria A
The Journal of cell biology 2009 Jun 1;185(5):859-74
The Journal of cell biology 2009 Jun 1;185(5):859-74
NDE1 and NDEL1: multimerisation, alternate splicing and DISC1 interaction.
Bradshaw NJ, Christie S, Soares DC, Carlyle BC, Porteous DJ, Millar JK
Neuroscience letters 2009 Jan 16;449(3):228-33
Neuroscience letters 2009 Jan 16;449(3):228-33
Cenp-F links kinetochores to Ndel1/Nde1/Lis1/dynein microtubule motor complexes.
Vergnolle MA, Taylor SS
Current biology : CB 2007 Jul 3;17(13):1173-9
Current biology : CB 2007 Jul 3;17(13):1173-9
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- A2780 cells were subjected to SDS PAGE followed by western blot with 10233-1-AP(NDE1 antibody) at dilution of 1:1500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using NDE1 antibody 10233-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-NDE1 (IP:10233-1-AP, 3ug; Detection:10233-1-AP 1:1500) with HEK-293 cells lysate 4500ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human breast cancer using 10233-1-AP(NDE1 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human breast cancer using 10233-1-AP(NDE1 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue