Antibody data
- Antibody Data
- Antigen structure
- References [24]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
Reference
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- Product number
- 14851-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14851-1-AP, RRID:AB_2261175
- Product name
- CUL4A-Specific antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated CUL4A-Specific antibody (Cat. #14851-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Proapoptotic effect of WS-299 induced by NOXA accumulation and NRF2-counterbalanced oxidative stress damage through targeting RBX1-UBE2M interaction in gastric cancers.
PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer.
Genetic Modulation of BET1L Confers Colorectal Cancer Susceptibility by Reducing miRNA Binding and m6A Modification.
Widespread dysregulation of mRNA splicing implicates RNA processing in the development and progression of Huntington's disease.
MOF negatively regulates estrogen receptor α signaling via CUL4B-mediated protein degradation in breast cancer.
AMPK induces degradation of the transcriptional repressor PROX1 impairing branched amino acid metabolism and tumourigenesis.
Discovery of Potent and Selective 2-(Benzylthio)pyrimidine-based DCN1-UBC12 Inhibitors for Anticardiac Fibrotic Effects.
Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy.
Evaluation of HZX-960, a novel DCN1-UBC12 interaction inhibitor, as a potential antifibrotic compound for liver fibrosis.
Cardioprotective effect of MLN4924 on ameliorating autophagic flux impairment in myocardial ischemia-reperfusion injury by Sirt1.
DDB1 binds histone reader BRWD3 to activate the transcriptional cascade in adipogenesis and promote onset of obesity.
The Mediator subunit MED20 organizes the early adipogenic complex to promote development of adipose tissues and diet-induced obesity.
The E3 ubiquitin ligase Cul4b promotes CD4+ T cell expansion by aiding the repair of damaged DNA.
Negative regulation of AMPK signaling by high glucose via E3 ubiquitin ligase MG53.
Functional impacts of the ubiquitin-proteasome system on DNA damage recognition in global genome nucleotide excision repair.
Effect of CUL4A on the metastatic potential of lung adenocarcinoma to the bone.
Cul4a as a New Interaction Protein of PARP1 Inhibits Oxidative Stress-Induced H9c2 Cell Apoptosis.
Potent 5-Cyano-6-phenyl-pyrimidin-Based Derivatives Targeting DCN1-UBE2M Interaction.
Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2.
Cul4 E3 ubiquitin ligase regulates ovarian cancer drug resistance by targeting the antiapoptotic protein BIRC3.
Amyloid Precursor Protein (APP) May Act as a Substrate and a Recognition Unit for CRL4CRBN and Stub1 E3 Ligases Facilitating Ubiquitination of Proteins Involved in Presynaptic Functions and Neurodegeneration.
CSN6 drives carcinogenesis by positively regulating Myc stability.
AMBRA1 interplay with cullin E3 ubiquitin ligases regulates autophagy dynamics.
COP9 signalosome subunit 8 is essential for peripheral T cell homeostasis and antigen receptor-induced entry into the cell cycle from quiescence.
Ma T, Song Q, Cheng B, Guo E, Wang X, Li M, Dai M, Li S, Feng S, Yu B
Bioorganic chemistry 2024 Mar;144:107142
Bioorganic chemistry 2024 Mar;144:107142
PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer.
Xu L, Xiang W, Yang J, Gao J, Wang X, Meng L, Ye K, Zhao XH, Zhang XD, Jin L, Ye Y
Journal of experimental & clinical cancer research : CR 2024 Jan 11;43(1):17
Journal of experimental & clinical cancer research : CR 2024 Jan 11;43(1):17
Genetic Modulation of BET1L Confers Colorectal Cancer Susceptibility by Reducing miRNA Binding and m6A Modification.
Li S, Du M, Xu K, Ben S, Zhu T, Guo M, Xin J, Zhu L, Gu D, Zhang Z, Wang M
Cancer research 2023 Jul 5;83(13):2142-2154
Cancer research 2023 Jul 5;83(13):2142-2154
Widespread dysregulation of mRNA splicing implicates RNA processing in the development and progression of Huntington's disease.
Tano V, Utami KH, Yusof NABM, Bégin J, Tan WWL, Pouladi MA, Langley SR
EBioMedicine 2023 Aug;94:104720
EBioMedicine 2023 Aug;94:104720
MOF negatively regulates estrogen receptor α signaling via CUL4B-mediated protein degradation in breast cancer.
Zhang X, Yang Y, Li D, Wu Z, Liu H, Zhao Z, Zhu H, Xie F, Li X
Frontiers in oncology 2022;12:868866
Frontiers in oncology 2022;12:868866
AMPK induces degradation of the transcriptional repressor PROX1 impairing branched amino acid metabolism and tumourigenesis.
Wang Y, Luo M, Wang F, Tong Y, Li L, Shu Y, Qiao K, Zhang L, Yan G, Liu J, Ji H, Xie Y, Zhang Y, Gao WQ, Liu Y
Nature communications 2022 Nov 24;13(1):7215
Nature communications 2022 Nov 24;13(1):7215
Discovery of Potent and Selective 2-(Benzylthio)pyrimidine-based DCN1-UBC12 Inhibitors for Anticardiac Fibrotic Effects.
He ZX, An Q, Wei B, Zhou WJ, Wei BF, Gong YP, Zhang X, Gao G, Dong GJ, Huo JL, Zhang XH, Yang FF, Liu HM, Ma LY, Zhao W
Journal of medicinal chemistry 2022 Jan 13;65(1):163-190
Journal of medicinal chemistry 2022 Jan 13;65(1):163-190
Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy.
Meng Y, Qiu L, Zeng X, Hu X, Zhang Y, Wan X, Mao X, Wu J, Xu Y, Xiong Q, Chen Z, Zhang B, Han J
Signal transduction and targeted therapy 2022 Dec 9;7(1):388
Signal transduction and targeted therapy 2022 Dec 9;7(1):388
Evaluation of HZX-960, a novel DCN1-UBC12 interaction inhibitor, as a potential antifibrotic compound for liver fibrosis.
Zhou W, Dong G, Gao G, He Z, Xu J, Aziz S, Ma L, Zhao W
Biochemistry and cell biology = Biochimie et biologie cellulaire 2022 Aug 1;100(4):309-324
Biochemistry and cell biology = Biochimie et biologie cellulaire 2022 Aug 1;100(4):309-324
Cardioprotective effect of MLN4924 on ameliorating autophagic flux impairment in myocardial ischemia-reperfusion injury by Sirt1.
Zhang J, Cui J, Zhao F, Yang L, Xu X, Shi Y, Wei B
Redox biology 2021 Oct;46:102114
Redox biology 2021 Oct;46:102114
DDB1 binds histone reader BRWD3 to activate the transcriptional cascade in adipogenesis and promote onset of obesity.
Wang X, Wang HY, Hu GS, Tang WS, Weng L, Zhang Y, Guo H, Yao SS, Liu SY, Zhang GL, Han Y, Liu M, Zhang XD, Cen X, Shen HF, Xiao N, Liu CQ, Wang HR, Huang J, Liu W, Li P, Zhao TJ
Cell reports 2021 Jun 22;35(12):109281
Cell reports 2021 Jun 22;35(12):109281
The Mediator subunit MED20 organizes the early adipogenic complex to promote development of adipose tissues and diet-induced obesity.
Tang WS, Weng L, Wang X, Liu CQ, Hu GS, Yin ST, Tao Y, Hong NN, Guo H, Liu W, Wang HR, Zhao TJ
Cell reports 2021 Jul 6;36(1):109314
Cell reports 2021 Jul 6;36(1):109314
The E3 ubiquitin ligase Cul4b promotes CD4+ T cell expansion by aiding the repair of damaged DNA.
Dar AA, Sawada K, Dybas JM, Moser EK, Lewis EL, Park E, Fazelinia H, Spruce LA, Ding H, Seeholzer SH, Oliver PM
PLoS biology 2021 Feb;19(2):e3001041
PLoS biology 2021 Feb;19(2):e3001041
Negative regulation of AMPK signaling by high glucose via E3 ubiquitin ligase MG53.
Jiang P, Ren L, Zhi L, Yu Z, Lv F, Xu F, Peng W, Bai X, Cheng K, Quan L, Zhang X, Wang X, Zhang Y, Yang D, Hu X, Xiao RP
Molecular cell 2021 Feb 4;81(3):629-637.e5
Molecular cell 2021 Feb 4;81(3):629-637.e5
Functional impacts of the ubiquitin-proteasome system on DNA damage recognition in global genome nucleotide excision repair.
Sakai W, Yuasa-Sunagawa M, Kusakabe M, Kishimoto A, Matsui T, Kaneko Y, Akagi JI, Huyghe N, Ikura M, Ikura T, Hanaoka F, Yokoi M, Sugasawa K
Scientific reports 2020 Nov 12;10(1):19704
Scientific reports 2020 Nov 12;10(1):19704
Effect of CUL4A on the metastatic potential of lung adenocarcinoma to the bone.
Cao PP, Chen WJ, Pang HL, Shen WW, Xue P, Duan L, Xu YX, Liu LL, Zhang HL
Oncology reports 2020 Feb;43(2):662-670
Oncology reports 2020 Feb;43(2):662-670
Cul4a as a New Interaction Protein of PARP1 Inhibits Oxidative Stress-Induced H9c2 Cell Apoptosis.
Ye N, Zhang N, Zhang Y, Qian H, Wu B, Sun Y
Oxidative medicine and cellular longevity 2019;2019:4273261
Oxidative medicine and cellular longevity 2019;2019:4273261
Potent 5-Cyano-6-phenyl-pyrimidin-Based Derivatives Targeting DCN1-UBE2M Interaction.
Zhou W, Ma L, Ding L, Guo Q, He Z, Yang J, Qiao H, Li L, Yang J, Yu S, Zhao L, Wang S, Liu HM, Suo Z, Zhao W
Journal of medicinal chemistry 2019 Jun 13;62(11):5382-5403
Journal of medicinal chemistry 2019 Jun 13;62(11):5382-5403
Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2.
Sun H, Zhang J, Xin S, Jiang M, Zhang J, Li Z, Cao Q, Lou H
PLoS genetics 2019 Feb;15(2):e1007685
PLoS genetics 2019 Feb;15(2):e1007685
Cul4 E3 ubiquitin ligase regulates ovarian cancer drug resistance by targeting the antiapoptotic protein BIRC3.
Hu X, Meng Y, Xu L, Qiu L, Wei M, Su D, Qi X, Wang Z, Yang S, Liu C, Han J
Cell death & disease 2019 Feb 4;10(2):104
Cell death & disease 2019 Feb 4;10(2):104
Amyloid Precursor Protein (APP) May Act as a Substrate and a Recognition Unit for CRL4CRBN and Stub1 E3 Ligases Facilitating Ubiquitination of Proteins Involved in Presynaptic Functions and Neurodegeneration.
Del Prete D, Rice RC, Rajadhyaksha AM, D'Adamio L
The Journal of biological chemistry 2016 Aug 12;291(33):17209-27
The Journal of biological chemistry 2016 Aug 12;291(33):17209-27
CSN6 drives carcinogenesis by positively regulating Myc stability.
Chen J, Shin JH, Zhao R, Phan L, Wang H, Xue Y, Post SM, Ho Choi H, Chen JS, Wang E, Zhou Z, Tseng C, Gully C, Velazquez-Torres G, Fuentes-Mattei E, Yeung G, Qiao Y, Chou PC, Su CH, Hsieh YC, Hsu SL, Ohshiro K, Shaikenov T, Wang H, Yeung SC, Lee MH
Nature communications 2014 Nov 14;5:5384
Nature communications 2014 Nov 14;5:5384
AMBRA1 interplay with cullin E3 ubiquitin ligases regulates autophagy dynamics.
Antonioli M, Albiero F, Nazio F, Vescovo T, Perdomo AB, Corazzari M, Marsella C, Piselli P, Gretzmeier C, Dengjel J, Cecconi F, Piacentini M, Fimia GM
Developmental cell 2014 Dec 22;31(6):734-46
Developmental cell 2014 Dec 22;31(6):734-46
COP9 signalosome subunit 8 is essential for peripheral T cell homeostasis and antigen receptor-induced entry into the cell cycle from quiescence.
Menon S, Chi H, Zhang H, Deng XW, Flavell RA, Wei N
Nature immunology 2007 Nov;8(11):1236-45
Nature immunology 2007 Nov;8(11):1236-45
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 14851-1-AP(CUL4A-Specific antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using CUL4A antibody 14851-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line