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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-49716 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cullin 4A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody detects endogenous level of total Cullin 4A protein.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.9 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CUL4A was performed by loading Lane1: Human fetal muscle tissue lysate (40µg), Lane2: Human fetal brain tissue lysate (40µg) on to a 8% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a CUL4A antibody (Product # PA5-49716) at a 1/450 dilution for 1 minute, followed by Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CUL4A was performed by loading Lane1: Human fetal muscle tissue lysate (40µg), Lane2: Human fetal brain tissue lysate (40µg) on to a 8% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a CUL4A antibody (Product # PA5-49716) at a 1/450 dilution for 1 minute, followed by Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Cullin 4A in paraffin-embedded Human liver cancer tissue using (left) Cullin 4A Polyclonal Antibody (Product # PA5-49716) at a 1:22 dilution and (right) is treated with the synthetic peptide.
