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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Immunocytochemistry [3]
- Flow cytometry [1]
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- Product number
- MA5-34615 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cullin 4A Recombinant Rabbit Monoclonal Antibody (JU07-33)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: Hela, A431, HUVEC, human liver cance tissue, human breast cancer tissue, human colon cancer tissue, human kidney tissue, human cervix tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JU07-33
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
Submitted references Inhibition of cytoplasmic EZH2 induces antitumor activity through stabilization of the DLC1 tumor suppressor protein.
Tripathi BK, Anderman MF, Bhargava D, Boccuzzi L, Qian X, Wang D, Durkin ME, Papageorge AG, de Miguel FJ, Politi K, Walters KJ, Doroshow JH, Lowy DR
Nature communications 2021 Dec 3;12(1):6941
Nature communications 2021 Dec 3;12(1):6941
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cullin 4a in HUVEC cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Cullin 4a monoclonal antibody (Product # MA5-34615), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cullin 4a in HUVEC cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Cullin 4a monoclonal antibody (Product # MA5-34615), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cullin 4a in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Cullin 4a monoclonal antibody (Product # MA5-34615), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Cullin 4a in HeLa cells (green) compared with an unlabelled control (cells without incubation with primary antibody; red). Samples were incubated with Cullin 4a monoclonal antibody (Product # MA5-34615) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
