Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- NBP2-29678 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal MKK7/MEK7 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MEK7 [p Thr277] Antibody [NBP2-29678] - Recombinant MKK7b1 (14-542) was left untreated (Lane 1) or phosphorylated by MEKK1 (14-196) (Lane 2) and probed with anti-phospho-MKK7/SKK4 (Thr275/Ser277) (1:1000 dilution).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MKK7/MEK7 [p Thr277] Antibody [NBP2-29678] - (No peptide/Lane 1), A431 treated with Calyculin A and okadaic acid (Control peptide/Lane 2), A431 treated with Calyculin A and okadaic acid (Phosphopeptide (Thr275/Ser277)/Lane 3) lysates were probed Anti-Phospho-MKK7/SAPKK4 (Thr275/Ser277) (1:500 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Phospho-MKK7/SAPKK4 (Thr275/Ser277) (~47 kDa).