HPA001634

RBM22 antibody from Atlas Antibodies

Cwc2, FLJ10290, fSAP47, ZC3H16

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Enhanced validation

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for HPA001634

Antibody Data

Product number

HPA001634

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA001634, RRID:AB_1079746

Product name

Anti-RBM22

Provider product page

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

NTYNRQNWEDADFPILCQTCLGENPYIRMTKEKYG
KECKICARPFTVFRWCPGVRMRFKKTEVCQTCSKL
KNVCQTCLLDLEYGLPIQVRDAGLSFKDDMPKSDV
NKEYYTQNMEREISNSDGTRPVGMLGKATSTSDML
LKL


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C, Rexhepaj E, Singan V, Murphy R, Pepperkok R, Uhlén M, Simpson J, Lundberg E
Nature Methods 2013 February;10(4):315-323

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Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy
Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of Proteomics 2012 April;75(7):2236-2251

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla)

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissueLane 6: Human tonsil tissue

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus.

Sample type

HUMAN

Enhanced validation

Submitted by

55af80e3e0991

Enhanced method

Genetic validation

Main image

Experimental details

Confocal images of immunofluorescently stained human U-2 OS cells.The protein RBM22 is shown in green and the microtubules in red. The image to the left show cells transfected with control siRNA and the image to the right show cells where RBM22 has been downregulated with specific siRNA.

Sample type

U-2 OS cells

Primary Ab dilution

1:60

Secondary Ab

Secondary Ab

Secondary Ab dilution

1:800

Knockdown/Genetic Approaches Application

Immunocytochemistry

KD Reagent Type

siRNA

Downregulation

>75%

KD Reagent Provider

Thermo Fisher Scientific

KD Reagent Prod no

s31274

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Prod no 2

s31273

KD Reagent Page 2

KD Reagent Page 2

KD Reagent Prod no 3

s31272

KD Reagent Page 3

KD Reagent Page 3

Antibody Lot Number

R00766

Appendix

55b0acac11273.pdf

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Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows strong nuclear positivity in non-germinal and germinal center cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neurons.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows moderate to strong nuclear positivity in cells in tubules and glomeruli.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human Fallopian tube shows strong nuclear positivity in glandular cells.

Sample type

HUMAN