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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-31469 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RBM22 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, Raji, K562, THP-1. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (91%), Xenopus laevis (94%), Chicken (97%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RBM22 Polyclonal Antibody (Product # PA5-31469). Sample (30 µg of whole cell lysate). Lane A: Jurkat. Lane B: Raji. Lane C: K562. Lane D: THP-1. 10% SDS PAGE. RBM22 Polyclonal Antibody (Product # PA5-31469) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RBM22 Polyclonal Antibody detects RBM22 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RBM22 protein stained by RBM22 Polyclonal Antibody (Product # PA5-31469) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
