PAB27477

antibody from Abnova Corporation

Targeting: NSUN7 FLJ14001

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB27477

Provider

Abnova Corporation

Product name

NSUN7 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant NSUN7.

Antigen sequence

SEVQEVENLLNSFKIKLAAALARCRIKHDALSIYH
ILPETVRKQELRASTLPLYAWINTCKISPEEVYNN
LKRRGYNKVKSVLHIDDKVFAVDQHCYDVLIFPSH
LKNDLINIDLFKDYKLIFQDKSRSLA


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with NSUN7 polyclonal antibody (Cat # PAB27477) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with NSUN7 polyclonal antibody (Cat # PAB27477) at 1-4 ug/mL dilutions shows positivity in vesicles.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum with NSUN7 polyclonal antibody (Cat # PAB27477) shows moderate cytoplasmic positivity in glandular cells at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)