AF5888

antibody from Novus Biologicals

Targeting: SH2B3 IDDM20, LNK

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF5888

Provider

Novus Biologicals

Product name

Sheep Polyclonal LNK/SH2B3 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human LNK/SH2B3 in Western blots.

Reactivity

Human

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

LNK suppresses interferon signaling in melanoma.

Ding LW, Sun QY, Edwards JJ, Fernández LT, Ran XB, Zhou SQ, Scolyer RA, Wilmott JS, Thompson JF, Doan N, Said JW, Venkatachalam N, Xiao JF, Loh XY, Pein M, Xu L, Mullins DW, Yang H, Lin DC, Koeffler HP
Nature communications 2019 May 20;10(1):2230

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LNK (SH2B3): paradoxical effects in ovarian cancer.

Ding LW, Sun QY, Lin DC, Chien W, Hattori N, Dong XM, Gery S, Garg M, Doan NB, Said JW, Xiao JF, Yang H, Liu LZ, Meng X, Huang RY, Tang K, Koeffler HP
Oncogene 2015 Mar 12;34(11):1463-74

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human LNK/SH2B3 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, SH-SY5Y human neuroblastoma cell line, Ramos human Burkitt's lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human LNK/SH2B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5888) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LNK/SH2B3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.