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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [5]
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- Product number
- PA5-52154 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LNK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: FDPPKSSRPK LQAACSSIQE VRRCTRLEMP DNLYTFVLKV KDRTDIIFEV GDEQQLNSWM AELSECTGRG LESTEAEMHI PSALEPSTSS SPRGSTDSLN QGASPGGLLD PACQKTDHFL SCYPWFH Highest antigen sequence identity to the following orthologs: Mouse - 79%, Rat - 77%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references TGF-β1/SH2B3 axis regulates anoikis resistance and EMT of lung cancer cells by modulating JAK2/STAT3 and SHP2/Grb2 signaling pathways.
Wang LN, Zhang ZT, Wang L, Wei HX, Zhang T, Zhang LM, Lin H, Zhang H, Wang SQ
Cell death & disease 2022 May 19;13(5):472
Cell death & disease 2022 May 19;13(5):472
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LNK in human cell line HEL using a LNK Polyclonal Antibody (Product # PA5-52154).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of LNK in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a LNK Polyclonal Antibody (Product # PA5-52154).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LNK in human lymph node shows strong nuclear membrane positivity in reaction center cells and lymphoid cells outside reaction center. Samples were probed using a LNK Polyclonal Antibody (Product # PA5-52154).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 SH2B3 was diminished in lung cancer tissues and cells. A qRT-PCR analysis of SH2B3 mRNA levels in human lung cancer tissues ( n = 40). B IHC assay to examine SH2B3 protein levels in human lung cancer tissues. C Bioinformatic analysis of SH2B3 mRNA level in human lung cancer tissues from UALCAN database. D Bioinformatic analysis of SH2B3 mRNA level in lung cancer tissues from patients at different stages from UALCAN database. E qRT-PCR analysis of SH2B3 mRNA levels in cultured lung cancer cell lines. F Western blotting to examine SH2B3 protein levels in cultured lung cancer cell lines. *** P < 0.001 and ** P < 0.01.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 SH2B3 promoted anoikis but suppressed cell proliferation of lung cancer. A qRT-PCR analysis of SH2B3 mRNA levels in transfected cells. B Western blotting to examine SH2B3 protein levels in transfected cells. C Flow cytometry to measure anoikis resistance of transfected cancer cells. D Soft colony formation to assess the number of anoikis resistance of cancer cells. E CCK-8 assay to evaluate the proliferation rate of transfected cancer cells. *** P < 0.001, ** P < 0.01, and * P < 0.05.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 SH2B3 bound to JAK2 and suppressed JAK2/STAT3 signaling. A Immunoblotting for Flag, Myc, JAK2, and SH2B3 following immunoprecipitation with Flag antibody in transfected cells. B Immunoblotting for JAK2 following SH2B3 immunoprecipitation in cancer cells. C Western blotting to determine protein levels of p-JAK2, JAK2, p-STAT3, and STAT3 in transfected cancer cells. *** P < 0.001 and ** P < 0.01.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 SH2B3 bound to SHP2 to inhibit SHP2/Grb2/PI3K/AKT signaling. A Immunoblotting for Flag, Myc, SHP2, and SH2B3 following immunoprecipitation with Flag antibody in transfected cells. B Immunoblotting for SHP2 following SH2B3 immunoprecipitation in cancer cells. C Western blotting to determine protein levels of p-SHP2, SHP2, Grb2, p-PI3K, PI3K, p-AKT, and AKT in transfected cancer cells. *** P < 0.001, ** P < 0.01, and * P < 0.05.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 7 SH2B3 inhibited lung cancer metastasis in vivo. A The area of tumor nodules in the liver of each group. B H&E staining of metastatic lesions in the liver of each group. C qRT-PCR analysis of SH2B3 mRNA levels in the liver tissues of each group. D Western blotting to measure SH2B3 protein levels in the liver tissues of each group. E Western blotting to determine p-JAK2, JAK2, p-STAT3, and STAT3 in the liver tissues from each group of mice. F Western blotting to determine p-SHP2, SHP2, Grb2, p-PI3K, PI3K, p-AKT, and AKT in the liver tissues from each group of mice. *** P < 0.001 and ** P < 0.01.
