TA502762

antibody from OriGene

Targeting: SH2B3 IDDM20, LNK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA502762

Provider

OriGene

Proper citation

OriGene Cat#TA502762, RRID:AB_11126502

Product name

SH2B3 (LNK) mouse monoclonal antibody, clone OTI1E6 (formerly 1E6)

Antibody type

Monoclonal

Description

SH2B3 (LNK) mouse monoclonal antibody, clone OTI1E6 (formerly 1E6)

Host

Mouse

Conjugate

Unconjugated

Epitope

SH2B3

Isotype

IgG

Antibody clone number

OTI1E6

Vial size

100 µl

Concentration

0.6 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH2B3 (RC218359, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH2B3.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SH2B3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-SH2B3 mouse monoclonal antibody (TA502762) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SH2B3(RC218359 ).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC218359 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SH2B3 antibody(TA502762), and then analyzed by flow cytometry.

Validation comment

FC