NB100-2295

antibody from Novus Biologicals

Targeting: SH2B3 IDDM20, LNK

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Antibody Data

Product number

NB100-2295

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-2295, RRID:AB_2301884

Product name

Goat Polyclonal LNK/SH2B3 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

0.1 mg

Concentration

0.5 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Lnk inhibits myeloproliferative disorder-associated JAK2 mutant, JAK2V617F.

Gery S, Cao Q, Gueller S, Xing H, Tefferi A, Koeffler HP
Journal of leukocyte biology 2009 Jun;85(6):957-65

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Cloning and characterization of human Lnk, an adaptor protein with pleckstrin homology and Src homology 2 domains that can inhibit T cell activation.

Li Y, He X, Schembri-King J, Jakes S, Hayashi J
Journal of immunology (Baltimore, Md. : 1950) 2000 May 15;164(10):5199-206

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Cloning and characterization of human Lnk, an adaptor protein with pleckstrin homology and Src homology 2 domains that can inhibit T cell activation.

Li Y, He X, Schembri-King J, Jakes S, Hayashi J
Journal of immunology (Baltimore, Md. : 1950) 2000 May 15;164(10):5199-206

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: LNK/SH2B3 Antibody [NB100-2295] - NB100-2295 (0.3 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: LNK/SH2B3 Antibody [NB100-2295] - HEK293 overexpressing SH2B3 and probed with (mock transfection in first lane).