Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [4]
- Immunohistochemistry [4]
- Other assay [3]
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Validation data
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- Product number
- PA5-29959 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ACADVL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299, HeLa, HepG2, mouse liver. Predicted reactivity: Mouse (86%), Rat (88%), Zebrafish (81%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Structural basis for defective membrane targeting of mutant enzyme in human VLCAD deficiency.
Dynamic Regulation of Long-Chain Fatty Acid Oxidation by a Noncanonical Interaction between the MCL-1 BH3 Helix and VLCAD.
Characterization of the mammalian family of DCN-type NEDD8 E3 ligases.
Prew MS, Camara CM, Botzanowski T, Moroco JA, Bloch NB, Levy HR, Seo HS, Dhe-Paganon S, Bird GH, Herce HD, Gygi MA, Escudero S, Wales TE, Engen JR, Walensky LD
Nature communications 2022 Jun 27;13(1):3669
Nature communications 2022 Jun 27;13(1):3669
Dynamic Regulation of Long-Chain Fatty Acid Oxidation by a Noncanonical Interaction between the MCL-1 BH3 Helix and VLCAD.
Escudero S, Zaganjor E, Lee S, Mill CP, Morgan AM, Crawford EB, Chen J, Wales TE, Mourtada R, Luccarelli J, Bird GH, Steidl U, Engen JR, Haigis MC, Opferman JT, Walensky LD
Molecular cell 2018 Mar 1;69(5):729-743.e7
Molecular cell 2018 Mar 1;69(5):729-743.e7
Characterization of the mammalian family of DCN-type NEDD8 E3 ligases.
Keuss MJ, Thomas Y, Mcarthur R, Wood NT, Knebel A, Kurz T
Journal of cell science 2016 Apr 1;129(7):1441-54
Journal of cell science 2016 Apr 1;129(7):1441-54
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ACADVL was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: ACADVL Polyclonal Antibody (Product # PA5-29959) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ACADVL Polyclonal Antibody detects ACADVL protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ACADVL stained by ACADVL Polyclonal Antibody (Product # PA5-29959) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ACADVL was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: ACADVL Polyclonal Antibody (Product # PA5-29959) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ACADVL Polyclonal Antibody detects ACADVL protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ACADVL stained by ACADVL Polyclonal Antibody (Product # PA5-29959) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ACADVL was performed in paraffin-embedded human A549 xenograft tissue using ACADVL Polyclonal Antibody (Product # PA5-29959) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ACADVL Polyclonal Antibody detects ACADVL protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. ACADVL stained by ACADVL Polyclonal Antibody (Product # PA5-29959) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H358 xenograft, using ACADVL (Product # PA5-29959) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ACADVL Polyclonal Antibody detects ACADVL protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. ACADVL stained by ACADVL Polyclonal Antibody (Product # PA5-29959) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL