PA5-29959

antibody from Invitrogen Antibodies

Targeting: ACADVL ACAD6, LCACD, VLCAD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29959

Provider

Invitrogen Antibodies

Product name

ACADVL Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: H1299, HeLa, HepG2, mouse liver. Predicted reactivity: Mouse (86%), Rat (88%), Zebrafish (81%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.3 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Structural basis for defective membrane targeting of mutant enzyme in human VLCAD deficiency.

Prew MS, Camara CM, Botzanowski T, Moroco JA, Bloch NB, Levy HR, Seo HS, Dhe-Paganon S, Bird GH, Herce HD, Gygi MA, Escudero S, Wales TE, Engen JR, Walensky LD
Nature communications 2022 Jun 27;13(1):3669

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Dynamic Regulation of Long-Chain Fatty Acid Oxidation by a Noncanonical Interaction between the MCL-1 BH3 Helix and VLCAD.

Escudero S, Zaganjor E, Lee S, Mill CP, Morgan AM, Crawford EB, Chen J, Wales TE, Mourtada R, Luccarelli J, Bird GH, Steidl U, Engen JR, Haigis MC, Opferman JT, Walensky LD
Molecular cell 2018 Mar 1;69(5):729-743.e7

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Characterization of the mammalian family of DCN-type NEDD8 E3 ligases.

Keuss MJ, Thomas Y, Mcarthur R, Wood NT, Knebel A, Kurz T
Journal of cell science 2016 Apr 1;129(7):1441-54

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ACADVL using 30 µg of HeLa lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an ACADVL polyclonal antibody (Product # PA5-29959) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ACADVL was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ACADVL Polyclonal Antibody (Product # PA5-29959) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ACADVL Polyclonal Antibody (Product # PA5-29959). Sample (50 µg of whole cell lysate). Lane A: mouse liver. 7.5% SDS PAGE. ACADVL Polyclonal Antibody (Product # PA5-29959) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of ACADVL was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: ACADVL Polyclonal Antibody (Product # PA5-29959) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ACADVL Polyclonal Antibody detects ACADVL protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ACADVL stained by ACADVL Polyclonal Antibody (Product # PA5-29959) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of ACADVL was performed in paraffin-embedded human A549 xenograft tissue using ACADVL Polyclonal Antibody (Product # PA5-29959) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ACADVL Polyclonal Antibody detects ACADVL protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. ACADVL stained by ACADVL Polyclonal Antibody (Product # PA5-29959) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded H358 xenograft, using ACADVL (Product # PA5-29959) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ACADVL Polyclonal Antibody detects ACADVL protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. ACADVL stained by ACADVL Polyclonal Antibody (Product # PA5-29959) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL