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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-78273 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WAVE1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: mouse brain, rat brain Predicted Reactivity: Chimpanzee (100%), Bovine (100%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.73 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WAVE1 in various tissue extracts using 50 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with WAVE1 polyclonal antibody (Product # PA5-78273) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using WAVE1 Polyclonal Antibody (Product # PA5-78273). Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WAVE1 Polyclonal Antibody (Product # PA5-78273) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of WAVE1 was performed in DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: WAVE1 Polyclonal Antibody (Product # PA5-78273) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WAVE1 Polyclonal Antibody detects WAVE1 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: WAVE1 protein stained by WAVE1 Polyclonal Antibody (Product # PA5-78273) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WAVE1 Polyclonal Antibody detects WAVE1 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. WAVE1 Polyclonal Antibody (Product # PA5-78273) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WAVE1 Polyclonal Antibody detects WAVE1 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. WAVE1 Polyclonal Antibody (Product # PA5-78273) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
