PA5-51771

antibody from Invitrogen Antibodies

Targeting: AADAC CES5A1, DAC

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-51771

Provider

Invitrogen Antibodies

Product name

AADAC Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: PALQPLDVDL PSYQENSNFL FLSKSLMVRF WSEYFTTDRS LEKAMLSRQH VPVESSHLFK FINWSSLLPE RFIKGHVYNN PNYGSSELAK KYPGFLDVRA APLLADDNKL RGLPLTYVIT CQYDLLRDDG LMYVTRLRNT GV Highest antigen sequence identity to the following orthologs: Mouse - 73%, Rat - 69%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

Lot-Specific

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-AADAC Polyclonal Antibody (Product # PA5-51771) and a 45 kDa band corresponding to AADAC was observed in Hep G2, PC-3, DU 145 and HT-29 cell line and Mouse Liver but not in Mouse Ovary, Mouse Adipose, Mouse Skeletal Muscle, Mouse Lung and Mouse Brain. Fig. (a) Whole cell extracts (30 µg lysate) Hep G2 (Lane 1), PC-3 (Lane 2), DU 145 (Lane 3) and HT-29 (Lane 4); Fig. (b) Tissue extracts (30 µg lysate) of Mouse Liver (Lane 1), Mouse Ovary (Lane 2), Mouse Adipose (Lane 3), Mouse Skeletal Muscle (Lane 4), Mouse Lung (Lane 5) and Mouse Brain (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of AADAC in human duodenum tissue shows moderate positivity in glandular cells. Samples were probed using an AADAC Polyclonal Antibody (Product # PA5-51771).