MA5-55968

antibody from Invitrogen Antibodies

Targeting: IRAG2 JAW1, LRMP

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-55968

Provider

Invitrogen Antibodies

Product name

Lrmp Monoclonal Antibody (K52026_16E2)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Sequence of this protein is as follows: LSGCIRMNDD PSMEENGVER VCPESLLQSR EYSSLPLPRH TSSTDGTITS SDPGLEILNM ASCDLDRNSL CKKEEDTRSA SPTIEAQGTS PAHDNIAFQD STSKDKTILN LEAKEEPETI EEHKKEHASG DSVVSPLPVT TVKSVNLRQS ENTSANEKEV EAEFLRLSLG FKCDWFTLEK RVKLEERSRD LAEENLKKEI TNCLKLLESL TPLCEDDNQA QEIIKKLEKS IKFLSQCAAR VASRAEMLGA INQESRVSKA VEVMIQHVEN LKRMYAKEHA ELEELKQVLL QNERSFNPLE

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

K52026_16E2

Vial size

50 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Lrmp in 15 µg of Ramos. Sample was run on 6-18% SDS-PAGE under reducing conditions, blotted onto nitrocellulose membrane, and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Lrmp band was visualized using ECL Western Blotting Substrate. Incubation with primary Lrmp monoclonal antibody (Product # MA5-55968) at a dilution of 1 µg/mL was used.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Lrmp in 200 µg of Ramos lysate. Samples are as follows: Lane 1: Ramos lysate, Lane 2: Lrmp immunoprecipitated from Ramos lysate, Lane3: The same as Lane 2 but KT82 was used as IgG isotype control antibody. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE, blotted onto nitrocellulose membrane, and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. The isotype control antibody was KT82. Incubation of samples with Lrmp monoclonal antibody (Product # MA5-55968) at a dilution of 2.5 µg was used.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Lrmp in paraffin-embedded tonsil tissue. Sample was incubated with Lrmp monoclonal antibody (Product # MA5-55968) at a dilution of 7.5 µg/mL (RT, 1 hour). Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.