PA5-34423

antibody from Invitrogen Antibodies

Targeting: TOX KIAA0808, TOX1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34423

Provider

Invitrogen Antibodies

Product name

TOX Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is human colon tissue lysate.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human colon tissue lysate using a TOX polyclonal antibody (Product # PA5-34423) at (A) 1 and (B) 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TOX in human colon tissue lysate with TOX Polyclonal Antibody (Product # PA5-34423) at (A) 1 and (B) 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot was performed using Anti-TOX Polyclonal Antibody (Product # PA5-34423) and 65, 70 kDa bands corresponding to Thymocyte selection-associated high mobility group box protein TOX were observed. Nuclear enriched extracts (40 µg lysate) of MOLT-4 (Lane 1), Ramos (Lane 2), Daudi (Lane 3), Jurkat (Lane 4), SH-SY5Y (Lane 5) and tissue extract (40 µg lysate) of Mouse Thymus (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556). Relative expression was observed between Ramos and Daudi as expected (https://doi.org/10.1371/journal.pone.0229743).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of human colon tissue using a TOX polyclonal antibody (Product # PA5-34423) at a 20 µg/mL dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of TOX in human colon tissue with TOX Polyclonal Antibody (Product # PA5-34423) at 20 µg/mL.