Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-34423 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TOX Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is human colon tissue lysate. PA5-34423 can be used with blocking peptide PEP-1465.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human colon tissue lysate using a TOX polyclonal antibody (Product # PA5-34423) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TOX in human colon tissue lysate with TOX Polyclonal Antibody (Product # PA5-34423) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-TOX Polyclonal Antibody (Product # PA5-34423) and 65, 70 kDa bands corresponding to Thymocyte selection-associated high mobility group box protein TOX were observed. Nuclear enriched extracts (40 µg lysate) of MOLT-4 (Lane 1), Ramos (Lane 2), Daudi (Lane 3), Jurkat (Lane 4), SH-SY5Y (Lane 5) and tissue extract (40 µg lysate) of Mouse Thymus (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556). Relative expression was observed between Ramos and Daudi as expected (https://doi.org/10.1371/journal.pone.0229743).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of human colon tissue using a TOX polyclonal antibody (Product # PA5-34423) at a 20 µg/mL dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of TOX in human colon tissue with TOX Polyclonal Antibody (Product # PA5-34423) at 20 µg/mL.