PA5-47267

antibody from Invitrogen Antibodies

Targeting: CA8 CALS, CARP

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

PA5-47267

Provider

Invitrogen Antibodies

Product name

Carbonic Anhydrase VIII Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) CA2 and rhCA3 is observed and less than 1% cross-reactivity with rhCA1, rhCA4, and rhCA9 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.

Reactivity

Human, Mouse

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Carbonic Anhydrase VIII in human brain (cerebellum) tissue and mouse brain (cerebellum) tissue. Samples were incubated in Carbonic Anhydrase VIII polyclonal antibody (Product # PA5-47267) using a dilution of 0.5 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Carbonic Anhydrase VIII/CA8 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Carbonic Anhydrase VIII in 0.2 mg/mL lysates of human brain (cerebellum) tissue and mouse brain (cerebellum) tissue. Samples were incubated in Carbonic Anhydrase VIII polyclonal antibody (Product # PA5-47267) using a dilution of 5 µg/mL followed by HRP-conjugated Anti-Goat IgG at a dilution of 1:50. Specific bands were detected for Carbonic Anhydrase VIII/CA8 at approximately 41 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.