PA5-34559

antibody from Invitrogen Antibodies

Targeting: GABARAP ATG8A, MM46

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34559

Provider

Invitrogen Antibodies

Product name

GABARAP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is K562 cell lysate.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of K562 cell lysate using a GABARAP polyclonal antibody (Product # PA5-34559) at (A) 1 and (B) 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of GABARAP in HeLa cell lysate with GABARAP Polyclonal Antibody (Product # PA5-34559) at 1 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-GABARAP Rabbit Polyclonal Antibody (Product # PA5-34559) and 14 kDa band corresponding to GABARAP were observed in HeLa cell line tested. Membrane enriched extracts (30 µg lysate) of HeLa (Lane 1) and HeLa treated with 50 µM Chloroquine for 20 hours (Lane 2) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2 µg/ml) and detected by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Uncharacterized bands (*) at 40 kDa and 58 kDa were observed.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of GABARAP in human brain tissue with GABARAP Polyclonal Antibody (Product # PA5-34559) at 20 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of GABARAP in mouse brain tissue with GABARAP Polyclonal Antibody (Product # PA5-34559) at 20 µg/mL. Green: GABARAP Red: Phylloidin staining Blue: DAPI staining

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of GABARAP in mouse brain tissue with GABARAP Polyclonal Antibody (Product # PA5-34559) at 5 µg/mL.