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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- F46304 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- GABARAP Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 1-30 from the human protein was used as the immunogen for this GABARAP antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the GABARAP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of GABARAP antibody and Hela cells treated with 26S proteasome complex blocker MG132.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of GABARAP antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the GABARAP gene (2).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent image of Chloroquine treated U251 cells stained with GABARAP antibody. Alexa Fluor 488 secondary (green) was used. Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). GABARAP immunoreactivity is localized to autophagic vacuoles in the cytoplasm.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- GABARAP antibody testing of mouse MEFs and mouse motor neurons. Antibodies used were GABARAP antibody (AF488 conjugated) and anti-Tuj1 (AF647 conjugated).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human hepatocarcinoma tissue stained with GABARAP antibody
