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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- LS-C773294 - Provider product page
- Provider
- LSBio
- Product name
- GABARAP Antibody (Atto 700) LS-C773294
- Antibody type
- Polyclonal
- Description
- Peptide Affinity Purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Near infrared dye
- Storage
- Store at -20°C.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAP Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAP Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Endomembrane System, Cytoplasm, Golgi Apparatus Membrane, Cytoplasmic Vesicle. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABARAP Antibody (D) Composite.
