AP02198SU-N

antibody from Acris Antibodies GmbH

Targeting: PTMA TMSA

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP02198SU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP02198SU-N, RRID:AB_1621691

Product name

anti Prothymosin alpha (75-109)

Antibody type

Polyclonal

Antigen

Synthetic Human Prothymosin alpha (aa 75-109) KLH conjugated

Reactivity

Human, Rat

Host

Rabbit

Vial size

0.1 ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. Western Blot analysis of AP02198SU specificity. Extracts of 231 and MCF-7 cells were separated by SDS-PAGE and immunoblotted with AP02198SU (2 µg/ml). AP02198SU Detects only Prothymosin alpha in cells extracts. Martini PG et al. (2000) Mol Cell Biol 20(17):6224-32.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 2. Immunohistochemistry of Prothymosin alpha staining in Paraffin sections of Rat testis. The sections were incubated with AP02198SU (1/1000)and detected using ABC method. The sections were counterstained with hematoxylin. Image A: AP02198SU stains Lydig cells (arrowhead), pachytene primary spermatozoides (arrow), and newly formed spermatids (asterisks). Image B: Control section without primary antibody. Scale Bar = 20 µm. Ferrara D et al. (2010) J. Cell. Physiol. 224(2):362-8.