PA5-66077

antibody from Invitrogen Antibodies

Targeting: TEAD1 AA, TCF13, TEF-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-66077

Provider

Invitrogen Antibodies

Product name

TEF1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: VSATAIHNKLG LPGIPRPTFP GAPGFWPGMI QTGQPGSSQD VKPFVQQAYP IQPAVTAPIP GFEPASA

Concentration

0.05 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TEF1 in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue. Samples were probed using a TEF1 Polyclonal Antibody (Product # PA5-66077).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TEF1 in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue. Samples were probed using a TEF1 Polyclonal Antibody (Product # PA5-66077).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-TEF1 Polyclonal Antibody (Product # PA5-66077) and a 50 kDa band corresponding to TEF1 was observed across all cell lines tested except for HepG2 and U-937, which are reported to be negative, along with an uncharacterized band (*) at ~36 kDa. Nuclear enriched extracts (60 µg lysate) of HeLa (Lane 1), SiHa (Lane 2), BeWo (Lane 3), Hep G2 (Lane 4) and U-937 (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of TEF1 in human cell line A549 shows localization to nucleoplasm. Samples were probed using a TEF1 Polyclonal Antibody (Product # PA5-66077).

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin Immunoprecipitation (ChIP) assay of endogenous TEF1 protein using TEF1 Antibody: ChIP was performed using Anti-TEF1 Polyclonal Antibody (Product # PA5-66077, 0.5 µg) on sheared chromatin from HeLa cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to transcription start site of CTGF, a region upstream of CTGF promoter and LATS2 (Active) and NF2, ACTB and SAT2 satellite repeats (Inactive). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.