MA5-27786

antibody from Invitrogen Antibodies

Targeting: TEAD1 AA, TCF13, TEF-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27786

Provider

Invitrogen Antibodies

Product name

TEF1 Monoclonal Antibody (GT13112)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Control: HeLa NE Predicted Reactivity: Pig (100%), Chicken (100%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

GT13112

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TEF1 in whole cell lysate using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with TEF1 monoclonal antibody (Product # MA5-27786) using a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using TEF1 Monoclonal Antibody (GT13112) (Product # MA5-27786). HeLa whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TEF1 Monoclonal Antibody (GT13112) (Product # MA5-27786) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using TEF1 Monoclonal Antibody (GT13112) (Product # MA5-27786) and a~48 kDa band corresponding to TEAD1 was observed across all the cell lines tested along with an uncharacterized band (*) at 30 kDa. Whole cell extracts (30 µg lysate) of A431 (Lane 1), HeLa (Lane 2), U2OS (Lane 3), BeWo (Lane 4), HEL 92.1.7 (Lane 5), U937 (Lane 6) and MOLT4 (Lane 7) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TEF1 Monoclonal Antibody (GT13112) detects TEF1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TEF1 protein stained by TEF1 Monoclonal Antibody (GT13112) (Product # MA5-27786) diluted at 1:400. Red: Phalloidin, a F-actin marker, diluted at 1:200.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of TEF1 was performed in HeLa nuclear extracts using 5 µg of TEF1 Monoclonal Antibody (GT13112) (Product # MA5-27786). Samples were transferred to a membrane and probed with TEF1 Monoclonal Antibody (GT13112) as a primary antibody and an HRP-conjugated anti-Mouse IgG was used as a secondary antibody.