PA5-120213

antibody from Invitrogen Antibodies

Targeting: TEAD1 AA, TCF13, TEF-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120213

Provider

Invitrogen Antibodies

Product name

TEF1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive test controls include: 293T, HeLa, Mouse skeletal muscle, Mouse heart, Rat heart. The target is usually found in the following locations: Nucleus. Immunogen sequence: APSVPAWQGR SIGTTKLRLV EFSAFLEQQR DPDSYNKHLF VHIGHANHSY SDPLLESVDI RQIYDKFPEK KGGLKELFGK GPQNAFFLVK FWADLNCNIQ D

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.16 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TEF1 in various cell lines using a TEF1 Polyclonal antibody (Product # PA5-120213) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 180s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TEF1 in various cell lines using a TEF1 Polyclonal antibody (Product # PA5-120213) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 180s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of TEF1 in MCF7 cells using a TEF1 Polyclonal antibody (Product # PA5-120213).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of TEF1 in human placenta using a Polyclonal antibody (Product # PA5-120213) at a dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol..

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of TEF1 in human placenta using a Polyclonal antibody (Product # PA5-120213) at a dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol..

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of TEF1 in human placenta using a Polyclonal antibody (Product # PA5-120213) at a dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol..

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ChIP analysis of TEF1 in extracts of HeLa cells using a TEF1 Polyclonal antibody (Product # PA5-120213) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. A histogram was constructed by the ratios of the immunoprecipitated DNA to the input.