Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- Ab130656 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant TEF1/TEAD-1 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human TEF1/TEAD-1 Antibody, Recombinant, could be used for WB and so on.ApplicationWB: 1/500-1/1000Protein FunctionTranscription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif.
- Reactivity
- Human, Rat, Hamster
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 411-426
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 1 mg/ml
- Storage
- Store at 4_ short term (1-2 weeks). Store at -20_ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
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- Aladdin Scientific (provider)
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- Experimental details
- Recombinant TEF1/TEAD-1 Antibody (Ab130656) - Western Blot All lanes: Recombinant TEF1/TEAD-1 Antibody (Ab130656) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution Predicted band size: 48 kDa Observed band size: 50 kDa Exposure time: 33.9 s