Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [6]
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Validation data
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- Product number
- NBP1-90149 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-90149, RRID:AB_11045430
- Product name
- Rabbit Polyclonal Annexin A6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat Annexin A6 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Comparative study of human and mouse postsynaptic proteomes finds high compositional conservation and abundance differences for key synaptic proteins.
Cell surface annexins regulate ADAM-mediated ectodomain shedding of proamphiregulin.
BayƩs A, Collins MO, Croning MD, van de Lagemaat LN, Choudhary JS, Grant SG
PloS one 2012;7(10):e46683
PloS one 2012;7(10):e46683
Cell surface annexins regulate ADAM-mediated ectodomain shedding of proamphiregulin.
Nakayama H, Fukuda S, Inoue H, Nishida-Fukuda H, Shirakata Y, Hashimoto K, Higashiyama S
Molecular biology of the cell 2012 May;23(10):1964-75
Molecular biology of the cell 2012 May;23(10):1964-75
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Annexin A6 Antibody [NBP1-90149] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Annexin A6 Antibody [NBP1-90149] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Annexin A6 Antibody [NBP1-90149] - Analysis in human cell lines SK-MEL-30 and A-431 using anti-ANXA6 antibody. Corresponding ANXA6 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Annexin A6 Antibody [NBP1-90149] - Staining of human pancreas shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Annexin A6 Antibody [NBP1-90149] - Staining in human appendix and pancreas tissues using anti-ANXA6 antibody. Corresponding ANXA6 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Annexin A6 Antibody [NBP1-90149] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Annexin A6 Antibody [NBP1-90149] - Staining of human pancreas shows strong membranous positivity in islets of Langerhans.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Annexin A6 Antibody [NBP1-90149] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Annexin A6 Antibody [NBP1-90149] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.