Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-27462 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Annexin A6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299, NIH-3T3. Predicted reactivity: Mouse (95%), Rat (95%), Chicken (80%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.179 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references iTRAQ-based quantitative proteomics reveals biomarkers/pathways in psoriasis that can predict the efficacy of methotrexate.
Yan KX, Meng Q, He H, Zhu HW, Wang ZC, Han L, Huang Q, Zhang ZH, Yawalkar N, Zhou H, Xu JX
Journal of the European Academy of Dermatology and Venereology : JEADV 2022 Oct;36(10):1784-1795
Journal of the European Academy of Dermatology and Venereology : JEADV 2022 Oct;36(10):1784-1795
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Annexin A6 Polyclonal Antibody (Product # PA5-27462). Sample (30 µg of whole cell lysate). A: NIH-3T3. 7.5% SDS PAGE. Annexin A6 Polyclonal Antibody (Product # PA5-27462) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Annexin A6 Polyclonal Antibody (Product # PA5-27462). Sample (30 µg of whole cell lysate). A: H1299. 7.5% SDS PAGE. Annexin A6 Polyclonal Antibody (Product # PA5-27462) diluted at 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Annexin A6 Polyclonal Antibody detects ANXA6 protein at cytosol on human breast by immunohistochemical analysis. Sample: Paraffin-embedded breast. Annexin A6 Polyclonal Antibody (Product # PA5-27462) dilution: 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 5 Figure Validation of STAT1 and ANXA6 proteins by Western blot. (a) The PASI score in nine GRs at baseline, 4 W, and 8 W. (b) Western blot analysis of expression levels of STAT1 and ANXA6 in GRs at baseline (0 W) and week 8 (8 W) after MTX treatment. (c) The expression of STAT1 and ANXA6 protein was significantly downregulated after MTX treatment (paired t -test, ** P < 0.01). (d) The PASI score in 9 NRs at baseline, 4 W and 8 W. (e) Western blot analysis of expression levels of STAT1 and ANXA6 in NRs at baseline (0 W) and week 8 (8 W) after MTX treatment. (f) The expression of STAT1 and ANXA6 protein in NR was not significantly downregulated after MTX treatment (paired t -test, P > 0.05). GR, good-responder; NR, non-responder; ns, no significance.