Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- HPA040665 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA040665, RRID:AB_10964282
- Product name
- Anti-BCL2L14
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human BCL2L14, Gene description: BCL2-like 14 (apoptosis facilitator), Alternative Gene Names: BCL-G, BCLG, Validated applications: WB, IHC, ICC, Uniprot ID: Q9BZR8, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Human BCL-G regulates secretion of inflammatory chemokines but is dispensable for induction of apoptosis by IFN-γ and TNF-α in intestinal epithelial cells
Woznicki J, Flood P, Bustamante-Garrido M, Stamou P, Moloney G, Fanning A, Zulquernain S, McCarthy J, Shanahan F, Melgar S, Nally K
Cell Death & Disease 2020;11(1)
Cell Death & Disease 2020;11(1)
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and BCL2L14 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408531).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human rectum and pancreas tissues using Anti-BCL2L14 antibody. Corresponding BCL2L14 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol