PA5-34706

antibody from Invitrogen Antibodies

Targeting: ARNTL bHLHe5, BMAL1, JAP3, MOP3, PASD3

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34706

Provider

Invitrogen Antibodies

Product name

BMAL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: BMAL1 tansfected 293T cell, mouse brain. Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (96%), Pig (99%), Chicken (97%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%). IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

0.65 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BMAL1 Polyclonal Antibody detects BMAL1 protein at nucleus by immunofluorescent analysis.Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BMAL1 protein stained by BMAL1 Polyclonal Antibody (Product # PA5-34706) diluted at 1:500.Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Arntl was performed using 70% confluent log phase NIH:OVCAR-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with BMAL1 Polyclonal Antibody (Product # PA5-34706) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731), (1:2500), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained withRhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents the low expression model Hep G2. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BMAL1 Polyclonal Antibody detects BMAL1 protein at nucleus by immunofluorescent analysis.Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BMAL1 protein stained by BMAL1 Polyclonal Antibody (Product # PA5-34706) diluted at 1:500.Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Arntl was performed using 70% confluent log phase NIH:OVCAR-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with BMAL1 Polyclonal Antibody (Product # PA5-34706) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731), (1:2500), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained withRhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents the low expression model Hep G2. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.