R32634

antibody from NSJ Bioreagents

Targeting: ARNTL bHLHe5, BMAL1, JAP3, MOP3, PASD3

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R32634

Provider

NSJ Bioreagents

Product name

BMAL1 Antibody / Brain and muscle ARNT-like 1

Antibody type

Polyclonal

Description

This highly specific BMAL1 antibody is suitable for use in Western blot/Flow cytometry applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the BMAL1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HeLa and 2) HepG2 cell lysate with BMAL1 antibody at 0.5ug/ml. Predicted molecular weight ~69 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat lung and 2) human HeLa lysate with CD151 antibody at 0.5ug/ml. Predicted molecular weight: ~28 kDa (unmodified), ~30 kDa (glycosylated).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HeLa, 2) human HT-1080, 3) human MCF7, 4) human SH-SY5Y, 5) rat brain, 6) rat liver, 7) mouse brain and 8) mouse liver tissue lysate with BMAL1 antibody. Predicted molecular weight ~69 kDa.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human MCF7 cells with BMAL1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BMAL1 antibody.