PA1-46118

antibody from Invitrogen Antibodies

Targeting: ARNTL bHLHe5, BMAL1, JAP3, MOP3, PASD3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-46118

Provider

Invitrogen Antibodies

Product name

BMAL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Suggested positive control: antigen standard for ARNTL (transient overexpression lysate), reticulocyte lysate.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.0 mg/mL

Storage

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

References

Submitted references

Metabolic Profiling of Suprachiasmatic Nucleus Reveals Multifaceted Effects in an Alzheimer's Disease Mouse Model.

Eeza MNH, Singer R, Höfling C, Matysik J, de Groot HJM, Roβner S, Alia A
Journal of Alzheimer's disease : JAD 2021;81(2):797-808

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MOP3 on 3T3/L1 cell lysates using Product # PA1-46118.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of BMAL1 using a polyclonal antibody (Product # PA1-46118).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of BMAL1 in A) MCF7, B) NIH/3T3, C) PC12. Samples were incubated in BMAL1 polyclonal antibody (Product # PA1-46118).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of BMAL1 in 3T3/L1 cell lysates. Sample was incubated in BMAL1 polyclonal antibody (Product # PA1-46118).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of BMAL1 in mouse brain. Samples were incubated with BMAL1 polyclonal antibody (Product # PA1-46118) followed by using DAB with hematoxylin counterstain.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of BMAL1 in formalin-fixed paraffin-embedded tissue section of mouse brain. Samples were incubated in BMAL1 polyclonal antibody (Product # PA1-46118) using a dilution of 1:200 followed by HRP labeled anti-rabbit IgG secondary antibody and DAB reagent. Nuclei of cells were counter-stained with hematoxylin. This BMAL1 antibody generated a specific nuclear staining in most of the cells and a relatively weaker cytoplasmic signal was also observed.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of BMAL1 in HeLa cells. Samples were incubated in BMAL1 polyclonal antibody (Product # PA1-46118) using a dilution of 5 µg/mL for 30 minutes at room temperature. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to Alexa Fluor 647.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of BMAL1 in Jurkat cells and a matched isotype control. Samples were incubated in BMAL1 polyclonal antibody (Product # PA1-46118) using a dilution of 2.5 µg/mL for 30 minutes at room temperature followed by a Rabbit IgG (H+L) Cross-Adsorbed secondary antibody. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 3 Immunohistochemical analyses of Bmal1 and GFAP staining in SCN of Tg2576 (TG) and wild-type (WT) mice. A) Representative confocal images of Bmal1 and GFAP stained sections through SCN of 18-month-old WT and TG mice. Scale bar, 250mum (first and third column); 60mum (second and fourth column) and 20mum (in magnifications). B) Quantitative analysis of Bmal1 and GFAP staining in SCN of 18 months old WT and Tg2576 (TG) mice ( n = 6 per group). ** p < 0.01, * p < 0.05. GFAP, glial fibrillary acidic protein.