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Western blot
Flow cytometry
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [2]
- Other assay [1]
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- Product number
- PA1-46118 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BMAL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Suggested positive control: antigen standard for ARNTL (transient overexpression lysate), reticulocyte lysate.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.0 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references Metabolic Profiling of Suprachiasmatic Nucleus Reveals Multifaceted Effects in an Alzheimer's Disease Mouse Model.
Eeza MNH, Singer R, Höfling C, Matysik J, de Groot HJM, Roβner S, Alia A
Journal of Alzheimer's disease : JAD 2021;81(2):797-808
Journal of Alzheimer's disease : JAD 2021;81(2):797-808
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of BMAL1 in HeLa cells. Samples were incubated in BMAL1 polyclonal antibody (Product # PA1-46118) using a dilution of 5 µg/mL for 30 minutes at room temperature. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to Alexa Fluor 647.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of BMAL1 in Jurkat cells and a matched isotype control. Samples were incubated in BMAL1 polyclonal antibody (Product # PA1-46118) using a dilution of 2.5 µg/mL for 30 minutes at room temperature followed by a Rabbit IgG (H+L) Cross-Adsorbed secondary antibody. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 Immunohistochemical analyses of Bmal1 and GFAP staining in SCN of Tg2576 (TG) and wild-type (WT) mice. A) Representative confocal images of Bmal1 and GFAP stained sections through SCN of 18-month-old WT and TG mice. Scale bar, 250mum (first and third column); 60mum (second and fourth column) and 20mum (in magnifications). B) Quantitative analysis of Bmal1 and GFAP staining in SCN of 18 months old WT and Tg2576 (TG) mice ( n = 6 per group). ** p < 0.01, * p < 0.05. GFAP, glial fibrillary acidic protein.
