Antibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [2]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- NB100-2288 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2288, RRID:AB_10000794
- Product name
- Rabbit Polyclonal BMAL1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Circadian Clock Regulation of Developmental Time in the Kidney.
Circadian clock gene NPAS2 promotes reprogramming of glucose metabolism in hepatocellular carcinoma cells.
Spatiotemporal expression pattern of PERIOD 1 and PERIOD 2 in the mouse SCN is dependent on VIP receptor 2 signaling.
Transcriptional Basis for Rhythmic Control of Hunger and Metabolism within the AgRP Neuron.
The Diurnal Rhythm of Insulin Receptor Substrate-1 (IRS-1) and Kir4.1 in Diabetes: Implications for a Clock Gene Bmal1.
Hippocampal clock regulates memory retrieval via Dopamine and PKA-induced GluA1 phosphorylation.
BMAL1 suppresses ROS-induced endothelial-to-mesenchymal transition and atherosclerosis plaque progression via BMP signaling.
Pharmacological targeting of the mammalian clock reveals a novel analgesic for osteoarthritis-induced pain.
Haploinsufficiency of hnRNP U Changes Activity Pattern and Metabolic Rhythms.
Regulation of amyloid-β dynamics and pathology by the circadian clock.
Mapping the co-localization of the circadian proteins PER2 and BMAL1 with enkephalin and substance P throughout the rodent forebrain.
Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells.
Dissociation of Molecular and Endocrine Circadian Rhythms in Male Mice Lacking Bmal1 in the Adrenal Cortex.
PI3K-PTEN dysregulation leads to mTOR-driven upregulation of the core clock gene BMAL1 in normal and malignant epithelial cells.
Pancreatic β cell enhancers regulate rhythmic transcription of genes controlling insulin secretion.
Impaired glucocorticoid production and response to stress in Arntl-deficient male mice.
Differential effects of light and feeding on circadian organization of peripheral clocks in a forebrain Bmal1 mutant.
Involvement of Wee1 in the circadian rhythm-dependent intestinal damage induced by docetaxel.
Global but not gonadotrope-specific disruption of Bmal1 abolishes the luteinizing hormone surge without affecting ovulation.
Formation of invadopodia-like structures by synovial cells promotes cartilage breakdown in collagen-induced arthritis: involvement of the protein tyrosine kinase Src.
Proxisome proliferator-activated receptor-α mediates high-fat, diet-enhanced daily oscillation of plasminogen activator inhibitor-1 activity in mice.
Dan H, Ruan T, Sampogna RV
Cell reports 2020 May 19;31(7):107661
Cell reports 2020 May 19;31(7):107661
Circadian clock gene NPAS2 promotes reprogramming of glucose metabolism in hepatocellular carcinoma cells.
Yuan P, Yang T, Mu J, Zhao J, Yang Y, Yan Z, Hou Y, Chen C, Xing J, Zhang H, Li J
Cancer letters 2020 Jan 28;469:498-509
Cancer letters 2020 Jan 28;469:498-509
Spatiotemporal expression pattern of PERIOD 1 and PERIOD 2 in the mouse SCN is dependent on VIP receptor 2 signaling.
Hannibal J, Norn THB, Georg B, Fahrenkrug J
The European journal of neuroscience 2019 Oct;50(7):3115-3132
The European journal of neuroscience 2019 Oct;50(7):3115-3132
Transcriptional Basis for Rhythmic Control of Hunger and Metabolism within the AgRP Neuron.
Cedernaes J, Huang W, Ramsey KM, Waldeck N, Cheng L, Marcheva B, Omura C, Kobayashi Y, Peek CB, Levine DC, Dhir R, Awatramani R, Bradfield CA, Wang XA, Takahashi JS, Mokadem M, Ahima RS, Bass J
Cell metabolism 2019 May 7;29(5):1078-1091.e5
Cell metabolism 2019 May 7;29(5):1078-1091.e5
The Diurnal Rhythm of Insulin Receptor Substrate-1 (IRS-1) and Kir4.1 in Diabetes: Implications for a Clock Gene Bmal1.
Luo Q, Xiao Y, Alex A, Cummins TR, Bhatwadekar AD
Investigative ophthalmology & visual science 2019 May 1;60(6):1928-1936
Investigative ophthalmology & visual science 2019 May 1;60(6):1928-1936
Hippocampal clock regulates memory retrieval via Dopamine and PKA-induced GluA1 phosphorylation.
Hasegawa S, Fukushima H, Hosoda H, Serita T, Ishikawa R, Rokukawa T, Kawahara-Miki R, Zhang Y, Ohta M, Okada S, Tanimizu T, Josselyn SA, Frankland PW, Kida S
Nature communications 2019 Dec 18;10(1):5766
Nature communications 2019 Dec 18;10(1):5766
BMAL1 suppresses ROS-induced endothelial-to-mesenchymal transition and atherosclerosis plaque progression via BMP signaling.
Zhu M, Tang H, Tang X, Ma X, Guo D, Chen F
American journal of translational research 2018;10(10):3150-3161
American journal of translational research 2018;10(10):3150-3161
Pharmacological targeting of the mammalian clock reveals a novel analgesic for osteoarthritis-induced pain.
Das V, Kc R, Li X, Varma D, Qiu S, Kroin JS, Forsyth CB, Keshavarzian A, van Wijnen AJ, Park TJ, Stein GS, O-Sullivan I, Burris TP, Im HJ
Gene 2018 May 20;655:1-12
Gene 2018 May 20;655:1-12
Haploinsufficiency of hnRNP U Changes Activity Pattern and Metabolic Rhythms.
Lai B, Zou J, Lin Z, Qu Z, Song A, Xu Y, Gao X
The American journal of pathology 2018 Jan;188(1):173-183
The American journal of pathology 2018 Jan;188(1):173-183
Regulation of amyloid-β dynamics and pathology by the circadian clock.
Kress GJ, Liao F, Dimitry J, Cedeno MR, FitzGerald GA, Holtzman DM, Musiek ES
The Journal of experimental medicine 2018 Apr 2;215(4):1059-1068
The Journal of experimental medicine 2018 Apr 2;215(4):1059-1068
Mapping the co-localization of the circadian proteins PER2 and BMAL1 with enkephalin and substance P throughout the rodent forebrain.
Frederick A, Goldsmith J, de Zavalia N, Amir S
PloS one 2017;12(4):e0176279
PloS one 2017;12(4):e0176279
Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells.
Boström J, Sramkova Z, Salašová A, Johard H, Mahdessian D, Fedr R, Marks C, Medalová J, Souček K, Lundberg E, Linnarsson S, Bryja V, Sekyrova P, Altun M, Andäng M
PloS one 2017;12(12):e0188772
PloS one 2017;12(12):e0188772
Dissociation of Molecular and Endocrine Circadian Rhythms in Male Mice Lacking Bmal1 in the Adrenal Cortex.
Dumbell R, Leliavski A, Matveeva O, Blaum C, Tsang AH, Oster H
Endocrinology 2016 Nov;157(11):4222-4233
Endocrinology 2016 Nov;157(11):4222-4233
PI3K-PTEN dysregulation leads to mTOR-driven upregulation of the core clock gene BMAL1 in normal and malignant epithelial cells.
Matsumoto CS, Almeida LO, Guimarães DM, Martins MD, Papagerakis P, Papagerakis S, Leopoldino AM, Castilho RM, Squarize CH
Oncotarget 2016 Jul 5;7(27):42393-42407
Oncotarget 2016 Jul 5;7(27):42393-42407
Pancreatic β cell enhancers regulate rhythmic transcription of genes controlling insulin secretion.
Perelis M, Marcheva B, Ramsey KM, Schipma MJ, Hutchison AL, Taguchi A, Peek CB, Hong H, Huang W, Omura C, Allred AL, Bradfield CA, Dinner AR, Barish GD, Bass J
Science (New York, N.Y.) 2015 Nov 6;350(6261):aac4250
Science (New York, N.Y.) 2015 Nov 6;350(6261):aac4250
Impaired glucocorticoid production and response to stress in Arntl-deficient male mice.
Leliavski A, Shostak A, Husse J, Oster H
Endocrinology 2014 Jan;155(1):133-42
Endocrinology 2014 Jan;155(1):133-42
Differential effects of light and feeding on circadian organization of peripheral clocks in a forebrain Bmal1 mutant.
Izumo M, Pejchal M, Schook AC, Lange RP, Walisser JA, Sato TR, Wang X, Bradfield CA, Takahashi JS
eLife 2014 Dec 19;3
eLife 2014 Dec 19;3
Involvement of Wee1 in the circadian rhythm-dependent intestinal damage induced by docetaxel.
Obi-Ioka Y, Ushijima K, Kusama M, Ishikawa-Kobayashi E, Fujimura A
The Journal of pharmacology and experimental therapeutics 2013 Oct;347(1):242-8
The Journal of pharmacology and experimental therapeutics 2013 Oct;347(1):242-8
Global but not gonadotrope-specific disruption of Bmal1 abolishes the luteinizing hormone surge without affecting ovulation.
Chu A, Zhu L, Blum ID, Mai O, Leliavski A, Fahrenkrug J, Oster H, Boehm U, Storch KF
Endocrinology 2013 Aug;154(8):2924-35
Endocrinology 2013 Aug;154(8):2924-35
Formation of invadopodia-like structures by synovial cells promotes cartilage breakdown in collagen-induced arthritis: involvement of the protein tyrosine kinase Src.
Lauzier A, Charbonneau M, Harper K, Jilaveanu-Pelmus M, Dubois CM
Arthritis and rheumatism 2011 Jun;63(6):1591-602
Arthritis and rheumatism 2011 Jun;63(6):1591-602
Proxisome proliferator-activated receptor-α mediates high-fat, diet-enhanced daily oscillation of plasminogen activator inhibitor-1 activity in mice.
Hayashida S, Kuramoto Y, Koyanagi S, Oishi K, Fujiki J, Matsunaga N, Ikeda E, Ohdo S, Shimeno H, Soeda S
Chronobiology international 2010 Oct;27(9-10):1735-53
Chronobiology international 2010 Oct;27(9-10):1735-53
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BMAL1 Antibody [NB100-2288] - Analysis of BMAL1 in A) MCF7, B) NIH/3T3, C) PC12.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BMAL1 Antibody [NB100-2288] - Analysis of MOP3 on 3T3/L1 cell lysates.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: BMAL1 Antibody [NB100-2288] - Analysis of BMAL1 in mouse brain using DAB with hematoxylin counterstain.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BMAL1 Antibody [NB100-2288] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of rabbit anti-BMAL1 antibody. The staining was developed using HRP labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This BMAL1 antibody generated a specific nuclear staining in most of the cells and a relatively weaker cytoplasmic signal was also observed.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): BMAL1 Antibody [NB100-2288] - An intracellular stain was performed on HeLa cells with NB100-2288AF647(blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: BMAL1 Antibody [NB100-2288] - An intracellular stain was performed on Jurkat cells with NB100-2288 and a matched isotype control. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: BMAL1 Antibody [NB100-2288] - Analysis of BMAL1 in mouse cerebral cortex (left: wt, right: Bmal1 KO) using anti-BMAL1 antibody. Image from verified customer review.